FastQCFastQC Report
Wed 6 Sept 2023
SRR18677066_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18677066_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12581403
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTC6469025.141731808447754TruSeq Adapter, Index 3 (100% over 50bp)
CCCATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAAAAAAAAACAAA442570.35176522046070696No Hit
CCCATGTACACGGAGTCGAGCTCAACCCGCAACGCAAAAAAAAAACAAAA396150.3148694942845404No Hit
GGGATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAAAAAAAAACAAA393050.3124055401452445No Hit
GGGATGTACACGGAGTCGAGCTCAACCCGCAACGCAAAAAAAAAACAAAA369720.29386229818725307No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA180600.1435451992118844No Hit
CCCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG143990.11444669565071558No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)