FastQCFastQC Report
Wed 6 Sept 2023
SRR18677067_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18677067_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17880748
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCG3153181.76344971698052TruSeq Adapter, Index 7 (96% over 32bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCGCGTATGCCG1447820.8097088555803147TruSeq Adapter, Index 7 (96% over 32bp)
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA647180.3619423527472117No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA459710.25709774557529697No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA415680.2324734960752201No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA326120.18238610599511834No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA249970.13979840216975262No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)