FastQCFastQC Report
Wed 6 Sept 2023
SRR18677068_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18677068_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13399968
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTC5126043.825412120387153TruSeq Adapter, Index 2 (100% over 50bp)
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA339370.25326179883414646No Hit
GGGATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAAAAAAAAACAAA259070.19333628259410768No Hit
GGGATGTACACGGAGTCGAGCTCAACCCGCAACGCAAAAAAAAAACAAAA251780.18789597109485634No Hit
CCCATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAAAAAAAAACAAA213250.15914217108578171No Hit
CCCATGTACACGGAGTCGAGCTCAACCCGCAACGCAAAAAAAAAACAAAA194530.14517198847042023No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA164810.12299283102765618No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA148340.11070175689971798No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)