FastQCFastQC Report
Wed 6 Sept 2023
SRR18677069_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18677069_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26663453
Total Bases3.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA859080.3221938283837431No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA378300.14187959826508592No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA335170.1257038988911151No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA329220.12347237996519056No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA308230.11560018126684493No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCG297060.11141092640926889TruSeq Adapter, Index 20 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)