FastQCFastQC Report
Wed 6 Sept 2023
SRR18677070_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18677070_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15387290
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTC3870372.515303214536153TruSeq Adapter, Index 8 (100% over 50bp)
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA1592461.0349190793180605No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA1042990.6778256600090075No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAACAAAAAAAAAAGAT770710.5008744229815646No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA620030.40294944723859755No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAACAAAAAAAAAAGA568400.3693957805435525No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA458460.29794720187895335No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA448330.29136384639530416No Hit
GGGGGGGGGATGCGTGCATTTATCAAAAAAAAAACAAAAAAAAAAGATCG387480.2518182214022092No Hit
GGGGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAA358990.23330294028383164No Hit
GGGGGGGGGGATGCGTGCATTTATCAAAAAAAAAACAAAAAAAAAAGATC338900.2202467101094475No Hit
CCCCGACTGCATAATTTGTGGTAGTGGGGAAAAAAAAAACAAAAAAAAAA317070.2060596765252361No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA292030.18978650561599864No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA288680.18760938410857272No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA286100.18593267560434618No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAACAAAA211040.13715215609766243No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA204340.13279791308281055No Hit
CCCCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAACAAAAAAAAA202990.13192056560966875No Hit
CCCCTAAACCATTCGTAGACGACCTGCTTAAAAAAAAAACAAAAAAAAAA190490.12379697789539289No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA180740.11746057947825769No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA168160.10928500080261047No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAAAA162340.10550265836284363No Hit
GGGCGACTGCATAATTTGTGGTAGTGGGGAAAAAAAAAACAAAAAAAAAA161970.10526220016650106No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)