FastQCFastQC Report
Wed 6 Sept 2023
SRR18677071_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18677071_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13092417
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAAAAAAAAACAAA5234173.997863801618907No Hit
GGGATGTACACGGAGTCGAGCTCAACCCGCAACGCAAAAAAAAAACAAAA4947023.7785383707225333No Hit
CCCATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAAAAAAAAACAAA4496883.434721029738054No Hit
CCCATGTACACGGAGTCGAGCTCAACCCGCAACGCAAAAAAAAAACAAAA4039133.0850911638393432No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTC3397372.5949142927543476TruSeq Adapter, Index 4 (100% over 50bp)
GGGGTACACGGAGTCGAGCTCAACCCGCAACGCGAAAAAAAAAACAAAAA859350.6563723107811186No Hit
GGGATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAAAAAAAAAAAAA740500.5655945727973681No Hit
GGGGTACACGGAGTCGAGCTCAACCCGCAACGCAAAAAAAAAACAAAAAA712940.5445442197571312No Hit
CCCATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAAAAAAAAAAAAA686680.5244868078980375No Hit
GGGATGTACACGGAGTCGAGCTCAACCCGCAACGCAAAAAAAAAAACAAA397560.3036566892117781No Hit
GGGATGTACACGGAGTCGAGCTCAACCCGCAACGCGTAAAAAAAAAACAA395650.30219782947640605No Hit
GGGATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAAAAAAAAAACAA355450.2714930329518224No Hit
GGGGGAGTCGAGCTCAACCCGCAACGCGAAAAAAAAAACAAAAAAAAAAG334000.25510950346295874No Hit
CCCATGTACACGGAGTCGAGCTCAACCCGCAACGCGTAAAAAAAAAACAA329230.2514661731290716No Hit
CCCATGTACACGGAGTCGAGCTCAACCCGCAACGCAAAAAAAAAAACAAA316600.24181936765381057No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA312390.2386037658287236No Hit
GGGATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAAAAAAAACAAAA309900.23670190156638No Hit
GGGGAGCTCAACCCGCAACGCGAAAAAAAAAACAAAAAAAAAAGATCGGA307580.23492988345849355No Hit
CCCATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAAAAAAAAAACAA302230.23084354859763478No Hit
GGGGGAGTCGAGCTCAACCCGCAACGCAAAAAAAAAACAAAAAAAAAAGA301000.2299040734800916No Hit
GGGATGTACACGGAGTCGAGCTCAACCCGCAACGCAAAAAAAAACAAAAA293590.22424430874757503No Hit
GGGGAGCTCAACCCGCAACGCAAAAAAAAAACAAAAAAAAAAGATCGGAA284690.2174464806612866No Hit
CCCATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAAAAAAAACAAAA259900.19851185613779335No Hit
GGGCACGGAGTCGAGCTCAACCCGCAACGCAAAAAAAAAACAAAAAAAAA250140.19105715927013325No Hit
CCCCCCGCAACGCGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACA247510.1890483628805896No Hit
GGGTACACGGAGTCGAGCTCAACCCGCAACGCAAAAAAAAAACAAAAAAA242390.18513770222870232No Hit
GGGCACGGAGTCGAGCTCAACCCGCAACGCGAAAAAAAAAACAAAAAAAA236520.18065419089538623No Hit
CCCATGTACACGGAGTCGAGCTCAACCCGCAACGCAAAAAAAAACAAAAA235640.17998204609584312No Hit
GGGAACGCGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACACGTCT233870.17863011848767116Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTACACGGAGTCGAGCTCAACCCGCAACGCAAAAAAAAAACAAAAAAA222940.1702817745569821No Hit
CCCCCCGCAACGCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACAC218850.16715782884092373No Hit
GGGGTCGAGCTCAACCCGCAACGCGAAAAAAAAAACAAAAAAAAAAGATC211820.16178830845366446No Hit
GGGTACACGGAGTCGAGCTCAACCCGCAACGCGAAAAAAAAAACAAAAAA210080.16045929487274962No Hit
CCCCACGGAGTCGAGCTCAACCCGCAACGCAAAAAAAAAACAAAAAAAAA207560.15853451658314885No Hit
CCCCGAGCTCAACCCGCAACGCAAAAAAAAAACAAAAAAAAAAGATCGGA204350.1560827156666336No Hit
GGGCAACCCGCAACGCGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGC204340.15607507765754786No Hit
GGGATGTACACGGAGTCGAGCTCAACCCGCAACGCGATAAAAAAAAAACA199910.1526914396325751No Hit
CCCCACGGAGTCGAGCTCAACCCGCAACGCGAAAAAAAAAACAAAAAAAA194620.14865093282623063No Hit
CCCTACACGGAGTCGAGCTCAACCCGCAACGCGAAAAAAAAAACAAAAAA185740.14186838075811364No Hit
GGGGCAACGCGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACACGT184130.14063866129531316No Hit
CCCCAACGCGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACACGTC181860.13890483323285532No Hit
GGGCAACGCGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACACGTC174970.1336422449727961No Hit
GGGGTCGAGCTCAACCCGCAACGCAAAAAAAAAACAAAAAAAAAAGATCG173150.13225212731919553No Hit
CCCCAACCCGCAACGCGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGC172700.13190841691033825No Hit
CCCCCGCAACGCGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACAC169220.1292503897485086No Hit
CCCATGTACACGGAGTCGAGCTCAACCCGCAACGCGATAAAAAAAAAACA167820.12818106847650818No Hit
GGGATGTACACGGAGTCGAGCTCAACCCGCAAAAAAAAAACAAAAAAAAA160810.12282682410742037No Hit
GGGCGAGCTCAACCCGCAACGCAAAAAAAAAACAAAAAAAAAAGATCGGA158930.12139087839930549No Hit
GGGACACGGAGTCGAGCTCAACCCGCAACGCAAAAAAAAAACAAAAAAAA155420.1187099372102187No Hit
CCCCAACCCGCAACGCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA151650.11583040778490328No Hit
CCCAACGCGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACACGTCT147810.11289741229598782Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCGAGCTCAACCCGCAACGCGAAAAAAAAAACAAAAAAAAAAGATCGG146390.11181281500581595No Hit
CCCCCGCAACGCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACACG142000.10845972901718606No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA138400.10571004574632782No Hit
GGGACACGGAGTCGAGCTCAACCCGCAACGCGAAAAAAAAAACAAAAAAA135790.1037165253749556No Hit
CCCCTCAACCCGCAACGCGAAAAAAAAAACAAAAAAAAAAGATCGGAAGA133070.10163898690364048No Hit
GGGATGTACACGGAGTCGAGCTCAACCCGCAACGTAAAAAAAAAACAAAA131200.10021067920461134No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)