FastQCFastQC Report
Wed 6 Sept 2023
SRR18677073_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18677073_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15744181
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTC4727493.002690327302513TruSeq Adapter, Index 2 (100% over 50bp)
GGGGGAGTTTGGCTGGGGCGGCACATCTGTAAAAAAAAAACAAAAAAAAA640900.40707103151316665No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA254450.16161526598303208No Hit
GGGGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGC219210.13923239322515413No Hit
GGGGAACGGGCTTGGCAGAATCAGCGGGGAAAAAAAAAACAAAAAAAAAA172280.10942455501496078No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)