FastQCFastQC Report
Wed 6 Sept 2023
SRR18677075_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18677075_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10650127
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTC141988213.332066368786025TruSeq Adapter, Index 8 (100% over 50bp)
CCCCTAAACCATTCGTAGACGACCTGCTTAAAAAAAAAACAAAAAAAAAA227340.21346224322019822No Hit
GGAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCT180560.16953788438391393TruSeq Adapter, Index 8 (98% over 50bp)
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA145550.1366650369521415No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGCCGTC143540.13477773551432767TruSeq Adapter, Index 8 (98% over 50bp)
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA132810.12470273828659507No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCACGTATGCCGTC114630.10763251931174155TruSeq Adapter, Index 8 (98% over 50bp)
GAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCT112850.1059611777399462TruSeq Adapter, Index 8 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)