FastQCFastQC Report
Wed 6 Sept 2023
SRR18677078_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18677078_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13325042
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTC9503717.132217669557815TruSeq Adapter, Index 4 (100% over 50bp)
GGGGGGGGGCTCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACACG275480.20673856037376842No Hit
CCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC152490.11443866368301128Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)