FastQCFastQC Report
Wed 6 Sept 2023
SRR18677080_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18677080_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17854718
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTC3972772.2250533444437486TruSeq Adapter, Index 9 (100% over 50bp)
GGGGGACGCATAGCTCAGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG256770.1438107283464236No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA194720.10905800920518599No Hit
GGGGGGCCGGTAGCTCAGGAAAAAAAAAACAAAAAAAAAAGATCGGAAGA189820.10631363654133323No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)