FastQCFastQC Report
Wed 6 Sept 2023
SRR18677081_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18677081_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10896555
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTC636040.5837074194550479TruSeq Adapter, Index 1 (100% over 50bp)
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA328100.30110433985787255No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAACAAAAAAAA184760.1695581768733329No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA150390.13801609774832505No Hit
CCCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG127750.11723888880476445No Hit
GGGCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG115100.10562971507967425No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)