FastQCFastQC Report
Wed 6 Sept 2023
SRR18677085_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18677085_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7021979
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCGTC390490.5560967926563153TruSeq Adapter, Index 16 (98% over 50bp)
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA228560.3254922864337817No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA106840.1521508395282868No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA103030.1467250186877517No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA99100.1411283058522391No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA94780.13497619403304964No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA84130.11980952947879793No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAACAAAAAAAAAAGAT77550.1104389517541992No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAACAAAAAAAAAAGA76120.10840248881405085No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)