FastQCFastQC Report
Wed 6 Sept 2023
SRR18677086_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18677086_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12815834
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCG377880.2948540063799203TruSeq Adapter, Index 18 (97% over 36bp)
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA246000.19195005178749974No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA134300.10479224371976104No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA131420.10254502360127324No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)