FastQCFastQC Report
Wed 6 Sept 2023
SRR18677087_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18677087_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5880563
Total Bases882 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCG404180.6873151431249015TruSeq Adapter, Index 19 (97% over 36bp)
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA265050.4507221502430975No Hit
GGGGGGGAGGTGGAGCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA244100.41509630965606525No Hit
GGGGAGCGCACGTGTTAGGACCCAAAAAAAAAACAAAAAAAAAAGATCGG153760.26147156318196063No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA132150.22472338107762813No Hit
GGGGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTAAAAAA122410.2081603411101964No Hit
GGGGGCGGGGCGCGGGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG122370.2080923204121782No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA111220.18913155083960498No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA104700.1780441770626384No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA99380.1689974242262178No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAAAAAA89430.15207727559419054No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA75940.12913729518755263No Hit
GGGGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCAAAAAAA74290.12633144139430189No Hit
GGGGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCAAAAAA67690.11510802622129888No Hit
GGGGGGGCGCGGGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCAC67230.11432578819408958No Hit
GGGGGGGAGGTGGAGTAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA67090.11408771575102587No Hit
CCCCTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCAAAAAAA64780.11015952044047482No Hit
CCCCTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAA64480.10964936520533833No Hit
GGGGGAGCCGGGCGTAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCAC62750.10670747001605119No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA60030.10208206255081359No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)