FastQCFastQC Report
Wed 6 Sept 2023
SRR18677089_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18677089_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7951592
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA339190.4265686670040415No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA212310.2670031359757895No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA174660.2196541271232226No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA140080.1761659803470802No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA108370.13628717368798599No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA92120.11585101448867095No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA91850.11551145984351309No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTC90260.11351186026647242TruSeq Adapter, Index 2 (100% over 50bp)
CCCCCCGTAGGGGTCACCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG87180.10963842209207918No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAAAAAAAAAACAAAAAAAAAAGAT82030.10316173163814239No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)