FastQCFastQC Report
Wed 6 Sept 2023
SRR18677090_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18677090_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5815061
Total Bases872.2 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA191640.32955802183330496No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA190510.3276147920030417No Hit
CCCCCCGTAGGGGTCACCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG128820.22152820065000178No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA112590.1936179173356909No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA108880.1872379326717295No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA98900.17007560195843174No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTC95530.16428030591596546TruSeq Adapter, Index 3 (100% over 50bp)
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA90920.15635261607745818No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA86540.1488204508946682No Hit
CCCCCCCCTCGGCTCCACCAAAAAAAAAACAAAAAAAAAAGATCGGAAGA63870.10983547722027336No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGCCGTC59100.10163263979518014TruSeq Adapter, Index 3 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)