FastQCFastQC Report
Wed 6 Sept 2023
SRR18677093_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18677093_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8136292
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA610230.7500099553949146No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA466250.5730497381362419No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA455050.5592842538099665No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA278960.3428588846123025No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTC222050.2729130173794156TruSeq Adapter, Index 6 (100% over 50bp)
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA197770.2430714138578114No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA197210.24238313964149763No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA193500.2378233229584189No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA169750.20863312182011168No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA152120.1869647746172335No Hit
GGGAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTC127990.15730753026071334TruSeq Adapter, Index 6 (98% over 50bp)
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAA122740.15085495948277175No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA122010.14995774487936275No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGCGTATGCCGTC110880.13627829483012655TruSeq Adapter, Index 6 (98% over 50bp)
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA108850.13378330079598913No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAAAAAAAAAACAAAAAAAAAAGAT99490.12227928889474468No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)