FastQCFastQC Report
Wed 6 Sept 2023
SRR18677094_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18677094_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7275045
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA958081.3169403075857262No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA552750.759789114706507No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA438200.6023330439880441No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA343380.4719970804304303No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA299420.4115713373594253No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA274280.37701485007996516No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA243740.335035728301337No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA180680.24835585209438565No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA132400.18199200142404617No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTC122170.1679302327339556TruSeq Adapter, Index 7 (100% over 50bp)
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA110730.15220524409127364No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA105860.14551112742257952No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA102440.14081012557310643No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAAAAAAAAAACAAAAAAAAAAGAT99110.13623283429861946No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAACAAAA97790.13441841253215614No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAAA95740.13160056054636088No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA95210.13087204271588698No Hit
CCCCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAAAAAAAAAACAA92660.1273669097579465No Hit
GGGGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAAAACAAAA89120.12250096047515859No Hit
CCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAACAA82300.11312644801509818No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA79570.10937389390718545No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGCCGTC77490.10651480506306146TruSeq Adapter, Index 7 (98% over 50bp)
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAA73880.10155263644417319No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)