FastQCFastQC Report
Wed 6 Sept 2023
SRR18677109.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18677109.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5528441
Total Bases829.2 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA2310464.179225210145138No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA1239832.2426394710552215No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA1058601.9148255358065684No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA642791.1626966806736294No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA440030.7959386742121332No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA408870.7395755874033927No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA347900.6292913318601031No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAACAAAA342290.619143805640686No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA319030.5770704616364722No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA306720.5548037864562541No Hit
CCCCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAACAAAAAAAA291180.5266945961800081No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAA285330.516112951191846No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAA265120.47955653320710123No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAACAAAA264780.4789415316180457No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA254650.4606181019205957No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAACAAAAAAAA250290.45273161095505954No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA250150.4524783750066248No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAA208880.37782803506449647No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA191740.346824719663283No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA185920.33629733952121404No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAACAA180580.32663819691663526No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAACAAAAAA174630.31587566910816267No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA168750.3052397592739074No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA164660.29784165192320944No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAACAAAAAA155640.28152602153120565No Hit
GGGCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAACAAAAAAAA150340.27193923205475107No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAAAAAAAAAACAA149000.26951540226259085No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAACAAAAAA146980.2658615692923195No Hit
GGGGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAAA144110.26067023234940917No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAAAAAAAAAACAAAAAAAAAAGAT133990.24236489093399025No Hit
CCCCGGGACCGGGGTCCGGTGCGGAGAGCCGTTAAAAAAAAAACAAAAAA130660.2363414930176518No Hit
CCCTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAACAA123920.2241499909287266No Hit
GGGTGGATAACTGTGGTAATTCTAGAGCTAATACATGAAAAAAAAAACAA118020.21347790453040918No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA114040.20627876828205274No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAA111270.2012683141594529No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTAAAAAAAAAACAAAAA111140.2010331664930493No Hit
GGGTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAACAA110340.19958610393056558No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAAAAAAAAACAAAA110140.19922433828994468No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAAAAAAAAAACAAAAAAAAAA110060.1990796320336963No Hit
GGGGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAACAAAAAAAAA108170.19566094672982853No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA107820.1950278568587419No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAA106820.19321902865563728No Hit
CCCTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAACAAAA103160.1865987174322743No Hit
CCCCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAAAAAAAAAACAA100820.18236605943700945No Hit
CCCCGGGGAGGTAGTGACGAAAAATAACAATACAGGAAAAAAAAAACAAA98260.1777354592370616No Hit
CCCCGACGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAACAAAAAAA97940.1771566342120681No Hit
GGGGGGCCGATCGCACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA96540.1746242747277216No Hit
GGGTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAACAAAA94580.17107897144963652No Hit
GGGGGGCCGATCGCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACA94520.17097044175745024No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAAA94170.17033735188636362No Hit
GGGGACGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAACAAAAAAAA88670.1603887967692881No Hit
CCCCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAAAAAAAAAACAAA87680.15859805684821454No Hit
CCCCCCCGAGTGTTACAGCCCTCCGGCCGCAAAAAAAAAACAAAAAAAAA83090.15029553539596424No Hit
GGGGTCGCGGCGTGGGAAATGTGGCGTACGGAAGAAAAAAAAAACAAAAA80290.14523081642727126No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAACAAAA79940.14459772655618464No Hit
CCCTGGATAACTGTGGTAATTCTAGAGCTAATACATGAAAAAAAAAACAA79720.14419978435150163No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAAAAAAAAAACAAA78210.14146845376481362No Hit
GGGGTCGCGGCGTGGGAAATGTGGCGTACGGAAAAAAAAAACAAAAAAAA75680.13689211841095889No Hit
GGGGATCGAGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACAC75430.13643991136018271No Hit
CCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAA75150.13593343946331343No Hit
GGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGAAAAAAAAAACAAA74630.13499284879769902No Hit
GGGTGGATAACTGTGGTAATTCTAGAGCTAATACATAAAAAAAAAACAAA73270.13253284244147673No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGAAAAAAAAAACAAAAAAA72760.13161034005789335No Hit
CCCCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAAAACAA72150.13050695485399952No Hit
GGGGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGAAAAAAAAAAC69980.12658179765326247No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAAAA69920.1264732679610762No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAACAAAAAA67320.12177031463300413No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCATCAAAAAAAAAACAAAAA66930.12106487163379334No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA65570.11860486527757101No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAACAAAAAA64640.11692265504868371No Hit
CCCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG64180.11609059407525557No Hit
GGGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAAAACAA62870.1137210291291885No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATAAAAAAAAAACAAAAAAA62580.11319646895028815No Hit
GGGTGGATAACTGTGGTAATTCTAGAGCTAATACAAAAAAAAAACAAAAA60920.11019381413313446No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAAAAA59850.1082583679558125No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAACAAAAA59260.10719115931598075No Hit
CCCCCCCGAGTGTTACAGCCCTCCGGCCGCAGAAAAAAAAAACAAAAAAA58070.10503865375428624No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAACAAAA57720.10440556388319963No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTC57410.10384482714023718TruSeq Adapter, Index 3 (100% over 50bp)
GGGTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAA57260.10357350290977149No Hit
CCCTCAAAAAGTAAGTTTAGATTATAGCCAAAAAAAAAACAAAAAAAAAA56370.10196364580900837No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)