FastQCFastQC Report
Wed 6 Sept 2023
SRR18677110.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18677110.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5080048
Total Bases762 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA1639733.2277844618791005No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA930201.8310850606136007No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA862161.697149318274158No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA621601.223610485570215No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA604141.1892407315836386No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA390330.7683588816483624No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA390040.76778802090059No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA339740.668773208442125No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA301560.5936164382698745No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA284290.559620696497356No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA259500.5108219449894962No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA249170.49048749145677367No Hit
GGGGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAACAA241560.4755073180410894No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAA227850.4485193840688119No Hit
GGGGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAAAAAAAAAACAAA224870.4426532977641156No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA223280.4395234060780528No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA211510.4163543336598395No Hit
CCCTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAACAA192400.3787365788669713No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAACAAAA176820.3480675773142301No Hit
CCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAC170480.3355873802767218No Hit
CCCGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA169820.3342881799542052No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA161400.3177135334154323No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAAAAAAAAAACAA160330.31560725410468565No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA157170.309386840439303No Hit
GGGTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAACAA132050.25993848877018483No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA131740.25932825831566947No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAACAAAA131240.25834401564709625No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTGAAAAAAAAAACAAAA129750.2554109724947481No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAA127930.25182832918114156No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAACAAAAAA126580.2491708739759939No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAACAAAA124290.24466304255392862No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA119030.23430880968053847No Hit
CCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAACA118390.23304897906476474No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAA112920.22228136427057382No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAACAAAAAA109730.21600189604507672No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAA109550.2156475686843904No Hit
GGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA109260.2150767079366179No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA104810.20631694818631635No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAACAAAA99080.19503752720446735No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAACAAAA95510.18801003455085466No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA95140.18728169497611047No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA93840.18472266403782012No Hit
CCCTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAACAAAA93630.18430928211701936No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA92300.18169119661861463No Hit
CCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAA91030.17919122024043868No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAA90810.17875815346626645No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAAAAAAAAACAAAA90250.17765580167746448No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA89500.17617943767460467No Hit
GGGGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAAAAAAAAAAAAA82850.16308901018258096No Hit
CCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA82800.16299058591572363No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTAAAAAAAAAACAAA82280.16196697354040748No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGAAAAAAAAAACAAA80570.15860086361388712No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAAAAAAAAAACAAAAAAAAAAGAT78700.15491979603342332No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAA77490.15253792877547612No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAACAAAAA75130.14789230337981057No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAACAAAAAA75020.14767576999272447No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA72720.1431482537172877No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA70030.13785302816036385No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAGAAAAAAAAAACAA69650.1371050037322482No Hit
CCCGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAAAAAAAAAACAA67240.13236095406972534No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAACAAAA66230.13037278387920745No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAACAAAAAA64790.1275381649937166No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAACAAA64500.12696730424594413No Hit
GGGTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAACAAAA62580.123187812398623No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAA62210.12245947282387884No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA61060.12019571468616044No Hit
CCCCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAAAAAAAAAACAA60730.11954611452490213No Hit
GGGTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAACA60080.11826659905575697No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTC59570.11726267153381227TruSeq Adapter, Index 7 (100% over 50bp)
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAAAAAAA58210.11458553147529316No Hit
CCCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAA55750.10974305754591294No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA55700.10964463327905562No Hit
CCCTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAA55680.1096052635723127No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA54880.10803047530259556No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAAAAAAAAAACAAAAAAAAAA53110.1045462562558464No Hit
CCCGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA53060.10444783198898908No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA52850.10403445006818832No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAACAAA52630.10360138329401612No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCAAAAAAAAAACAAAA51600.1015738433967553No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAACAAAAA51310.10100298264898283No Hit
CCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAACAAA51070.1005305461680677No Hit

[FAIL]Adapter Content

Adapter graph