FastQCFastQC Report
Wed 6 Sept 2023
SRR18677113_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18677113_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4806116
Total Bases720.9 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA1024222.131076320255275No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA648771.3498841892288909No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA580871.2086058680231606No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA551461.147413004596643No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA524441.0911929716219915No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA395250.8223896385355658No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAAA298990.6221031702106232No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAAA275740.5737273091202959No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA264460.5502572139332468No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAA251100.5224592997755361No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTC234800.488544179957371TruSeq Adapter, Index 5 (100% over 50bp)
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA191270.3979720839030935No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAACAAAAAAAA184200.38326166076723905No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAACAAAA173310.36060303163718893No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA150590.3133299321115013No Hit
CCCCTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAA146030.3038420212912048No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAAAAAAAAAACAAAAAAAAAAGAT144280.30020082744569626No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAAAAAAAAAACAAA144090.29980549782818394No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGAAAAAAAAAACAAAAAAA143420.29841144075590353No Hit
GGGGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAACAAAAAAAAA138690.28856981396204334No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA132740.2761897548873144No Hit
CCCCCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAG131330.27325599298893327No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTAAAAAAAAAACAAAAA123660.2572971605346188No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA116900.24323174887996876No Hit
GGGGGGCCGATCGCACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA116150.2416712372318937No Hit
GGGGGGCCGATCGCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACA112450.23397271310138995No Hit
CCCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG108670.22610773439509158No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGCCGTC102390.21304105019520958TruSeq Adapter, Index 5 (98% over 50bp)
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA99790.20763127648188268No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA96660.20111874120391598No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAACAAAAAA93910.19539686516097404No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAAA92170.19177647813743987No Hit
GGGGGCCCGTCTCGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACA91870.19115227347820984No Hit
CCCCCCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG91820.19104823936833817No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAAAAAAAAAACAAAAAAAAAA89540.18630428395818993No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAAAA88550.18424440858273083No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA86580.18014546465378697No Hit
CCCCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA79920.16628812121888026No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCTAAAAAAAAAACAAAAAA75000.15605116480750778No Hit
GGGGACGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAACAAAAAAAA72180.1501836410107455No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA66810.13901037761052792No Hit
GGGGAGCGTCGCTAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACAC65860.13703372952296616No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAACAA65360.13599338842424943No Hit
GGGGACGTACGCAGTTTTATCCGGTAAAAAAAAAACAAAAAAAAAAGATC64110.13339253567745762No Hit
CCCCCGCCTGGATACCGCAGCTAGGAAAAAAAAAACAAAAAAAAAAGATC63900.13295559241599664No Hit
GGGGATCGAGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACAC59970.12477851138008322No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA59160.12309315880016214No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAGAAAAAAAAAACAA58560.12184474948170206No Hit
CCCCTGAGCGTCGCTAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCAC58290.12128296528839504No Hit
GGGTGGATAACTGTGGTAATTCTAGAGCTAATACATGAAAAAAAAAACAA57750.12015939690178097No Hit
CCCCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAACAAAAAAAA57530.11970164681834562No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAAAAAAAAAACAA57260.11913986262503859No Hit
GGGGGCCCGTCTCGCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCAC56980.11855727160975722No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAACAAAAA54630.11366766844578866No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAAAAAAAAAACAA53170.11062987243753584No Hit
CCCCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTAAAAAAAAAACAAA53140.11056745197161283No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCCAAAAAAAAAACAAAAA52650.10954791769487045No Hit
GGGGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGC51950.10809144015666704No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGAAAAAAAAAACAAA51550.10725916727769368No Hit
GGGGGAATAATGGAATAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA51550.10725916727769368No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTAAAAAAAAAACAAAAAAAA50180.10440863266720987No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAACAAAAAA49940.10390926893982584No Hit
GGGGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAAA49240.10245279140162243No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAAA48640.10120438208316236No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)