FastQCFastQC Report
Wed 6 Sept 2023
SRR18677114_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18677114_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6517501
Total Bases977.6 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA1321412.0274795508278403No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA797371.2234290412843818No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA718211.1019714458041512No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA648130.9944455704724863No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA593940.9113002054008122No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA487070.7473263141808494No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAAA425360.6526427844046361No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAAA367080.563222007944456No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA344410.5284387374854258No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAACAAAAAAAA318180.4881932507566934No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAA296220.4544993548907779No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA201610.3093363545322049No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGAAAAAAAAAACAAAAAAA199540.3061602905776309No Hit
GGGGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAACAAAAAAAAA192340.2951131116051996No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAAAAAAAAAACAAA163230.2504487532874947No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAACAAAAAA163040.25015723050905553No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA141980.217844232014694No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAAAAAAAAAACAAAAAAAAAAGAT141740.21747599271561294No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA137790.2114153875849041No Hit
GGGGGCCCGTCTCGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACA136170.20892977231610704No Hit
CCCCTAAACCATTCGTAGACGACCTGCTTAAAAAAAAAACAAAAAAAAAA126690.19438432000240582No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA124910.19165321186755477No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA123540.18955117920196712No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA117950.1809742721942045No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAAA117370.18008436055475863No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTC115980.17795164128091426TruSeq Adapter, Index 6 (100% over 50bp)
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA103680.1590793772030108No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAAAA103570.15891060085759864No Hit
GGGGACGTACGCAGTTTTATCCGGTAAAAAAAAAACAAAAAAAAAAGATC99400.1525124430360655No Hit
GGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAACAAAAAAAAA98640.15134635192230889No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA94660.14523971687921491No Hit
GGGGACGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAACAAAAAAAA92870.14249326544023544No Hit
GGGGGGCCGATCGCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACA91010.13963941087235734No Hit
CCCCCGCCTGGATACCGCAGCTAGGAAAAAAAAAACAAAAAAAAAAGATC85450.13110853377697984No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCTAAAAAAAAAACAAAAAA85320.13090907082331096No Hit
GGGGAGCGTCGCTAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACAC85280.13084769760679746No Hit
CCCCCCGGGGAGGTAGTGACAAAAAAAAAACAAAAAAAAAAGATCGGAAG84870.130218622137534No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAAAAAAAAAACAAAAAAAAAA84640.12986572614258135No Hit
GGGGCAGTTAAAAAGCTCGTAGTTGGATCTTGAAAAAAAAAACAAAAAAA80490.12349825492930495No Hit
GGGGGCCCGTCTCGCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCAC79930.12263902989811586No Hit
GGGGATCGAGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACAC79660.12222476068664968No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAACAA79020.12124278922243358No Hit
CCCCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAACAAAAAAAA78890.12104332626876467No Hit
CCCCTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAA74710.11462982514310316No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA74580.11443036218943425No Hit
GGGGGCCCGAGGTGGGATCCCGAGGCCTAAAAAAAAAACAAAAAAAAAAG73280.11243573265274527No Hit
CCCCTGAGCGTCGCTAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCAC72790.1116839107504548No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAGAAAAAAAAAACAA70490.10815495080092816No Hit
CCCCCCGGGGAGGTAGTGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAG69060.10596085831057027No Hit
GGGGGGCCGATCGCACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA68520.10513231988763792No Hit
GGGGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGC66520.10206365906196256No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAACAAAAAA66460.10197159923719229No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)