FastQCFastQC Report
Wed 6 Sept 2023
SRR18677120_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18677120_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40999576
Total Bases6.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA11766892.870002850761188No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA7046631.7187080178585261No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA4456621.0869917288900743No Hit
CCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAC4347081.0602743794228506No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA4002150.9761442411014202No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA3740160.9122435802750741No Hit
CCCTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAACAA3055280.7451979503397791No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA2956500.7211050182567741No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTC2713130.6617458678109257TruSeq Adapter, Index 9 (100% over 50bp)
GGGTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAACAA2372480.5786596427241102No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA2290480.558659435892703No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA2262440.5518203407762071No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA2216100.5405177848668484No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA2038270.49714416558844415No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAAAAAAAAAACAA1919780.46824386671706064No Hit
CCCCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA1814580.44258506478213333No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAAAAAAAAACAAAAAA1705270.4159238134560221No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA1555130.3793039225576382No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA1394240.340062053324649No Hit
GGGGGGCCGATCGCACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA1335290.3256838558525581No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAACAAAA1333310.325200924029068No Hit
GGGGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAA1318880.3216813754366631No Hit
CCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAACAA1316470.32109356447978876No Hit
CCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAACA1240180.3024860549777393No Hit
CCCTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAACAAAA1067180.2602904966626972No Hit
CCCCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAAAAAAAAAACAA1035430.25254651413956086No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAA974270.23762928670286737No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA951000.23195361825205218No Hit
CCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAA928300.2264169756292114No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTAAAAAAAAAACAAAAA922420.22498281445642265No Hit
GGGGGCCGATCGCACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCAC865970.21121437938772827No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAA830190.20248745987031672No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAA829780.20238745883615966No Hit
CCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA829460.20230940924852492No Hit
GGGTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAACAAAA828140.20198745469953155No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAAAAAAAAACAAAA816300.1990996199570454No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAACAAAA800470.1952386044187384No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAA719720.17554327878902945No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA716800.17483107630186223No Hit
GGGGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAACAAAA708500.17280666512258566No Hit
GGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAC661660.1613821567325477No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCGCGTATGCCGTC655120.15978701828526226TruSeq Adapter, Index 9 (98% over 50bp)
GGGTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAACA651680.1589479852181886No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAAA639890.1560723457237704No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAAAA632830.15435037669657853No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA625370.15253084568484318No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAA624170.15223815973121282No Hit
CCCGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA598480.14597224127390976No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA586610.1430770893825829No Hit
CCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAAAAAAAAAAC575750.14042828150222822No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA575710.14041852530377388No Hit
GGGGATCGCACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACACGT542380.1322891729416909No Hit
GGGGATCGAGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACAC542350.13228185579285015No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA536690.13090135371156034No Hit
GGGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA507250.12372079164916243Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA501550.12233053336941827No Hit
GGGTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAA501250.12225736188101066No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA498180.1215085736496397No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAACAAAAAA497350.12130613253171203No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCCAAAAAAAAAACAAAAA491620.11990855710312712No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTGAAAAAAAAAACAAAA479780.117020722360641No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAACAAAAAA478000.11658657152942264No Hit
CCCTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAAAAAAAAAACAA472630.11527680188692684No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAAAAAAAAAACAAA469390.11448654981212487No Hit
GGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAA466550.11379385972186638No Hit
GGGGAGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACACGTCT460910.11241823573980374Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAA450640.10991333178665068No Hit
CCCTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAA450590.10990113653858274No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA444730.10847185346502121No Hit
GGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAACAAAA443030.10805721503071154No Hit
CCCCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACATAAAAAAAAA440860.1075279412645633No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGAAAAAAAAAACAAA440020.10732306109702207No Hit
CCCCTTTCGCCGAATCCCGGGGCCGAGGAAGAAAAAAAAAACAAAAAAAA439830.10727671915436393No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAACAAAA439420.10717671812020689No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAGAAAAAAAAAACAA437750.10676939683473799No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAAAAAAAAAACAAAAAAAAAAGAT416340.10154739161204986No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAA416240.10152300111591397No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)