FastQCFastQC Report
Wed 6 Sept 2023
SRR18677122_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18677122_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73712339
Total Bases11 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA24961653.3863597789238518No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA9669821.3118319308793063No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA6749770.9156906552646498No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTC6587300.8936495693075213TruSeq Adapter, Index 5 (100% over 50bp)
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA5828950.7907699143829908No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA5353600.726282746230587No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA5098900.6917295081356731No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA4443240.6027810350720251No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAA4438160.6020918695850908No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAAAA4253840.5770865580591602No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA3178700.43123038057441104No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA3157220.4283163501296574No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAAA2717140.3686139982615394No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAAA2469710.3350470265229272No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA2459610.3336768352989043No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA2287250.3102940472422127No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA1968180.26700821418785803No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA1909600.25906110508852526No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA1768140.23987028820235917No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA1687460.22892503790986743No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAA1653220.2242799539979324No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAACAAAAAAAA1638240.22224772978646085No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAAAA1585580.2151037426719019No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA1505020.20417477187910155No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAACAAAAAAAA1464970.1987414888571098No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAAAAAAAAAACAA1426530.19352662245597715No Hit
CCCCTAAACCATTCGTAGACGACCTGCTTAAAAAAAAAACAAAAAAAAAA1417460.1922961636043051No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA1362960.18490255749447865No Hit
GGGGATCGAGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACAC1355050.1838294671398231No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTGAAAAAAAAAACAAAA1322110.1793607444745445No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAGAAAAAAAAAACAA1301440.17655660065270753No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGAAAAAAAAAACAAA1288940.17486081943485743No Hit
GGGGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAA1287210.174626123314307No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAAA1276660.1731948839664415No Hit
GGGGAGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACACGTCT1226020.16632493509668714Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAAACAAAA1214270.16473090075190805No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAACAAAAAA1165430.1581051443775241No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA1074230.14573272461208972No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAAA1058020.14353363552878168No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAAAAAAAAAACAAA1047740.14213902505522177No Hit
GGGGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAACAAAAAAA1038720.14091534932842112No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAACAAAAAA1033920.1402641693407667No Hit
GGGGAGCGTCGCTAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACAC1027300.13936608360779326No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAACAAAAAA1004420.13626212566664042No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCTAAAAAAAAAACAAAAAA994170.13487158506800334No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAA959590.1301803759069428No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAACAAAA856380.11617864954739804No Hit
CCCCGCGACCTCAGATCAGACGTGGCGACCCGCTAAAAAAAAAACAAAAA853570.11579743792962532No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAAAAA853240.11575266930547408No Hit
CCCCTGAGCGTCGCTAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCAC839760.11392393884014454No Hit
GGGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC835510.11334737322607549Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGATTAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACACGTCTGA822800.11162310288376551Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCCTGATCGAGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCAC786340.10667684822754031No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAGAAAAAAAAAACA757000.10269651055300254No Hit
CCCCTAGACCATTCGTAGACGACCTGCTTAAAAAAAAAACAAAAAAAAAA746710.10130054345446832No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAGAAAAAAAAA741690.10061951771737972No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)