FastQCFastQC Report
Fri 30 Jun 2023
SRR1873532.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1873532.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21576375
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG1831410.8488033786954482No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1530550.7093638296516445Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG1333930.6182363812271524No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA1115380.5169450382652322No Hit
TTGAGTCTTAAACCAATTTTTTGAGGCCTCTGTAGGCACCATCAATAGAT999680.46332157278504843No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG932770.43231080290363877No Hit
ACGATTAAAGTCCTACGTGATCTGAGTCTGTAGGCACCATCAATAGATCG895570.41506972325054603No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA851290.3945472768247678No Hit
TCGACTGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAATAGATCGG722630.33491724165899045No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG704920.3267091900284455No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA687700.3187282386406428No Hit
ACATATACTAAAATTGGAACGATACAGAGAACTGTAGGCACCATCAATAG626160.2902063020317361No Hit
AAGCTGAACAAAATGAGTGAAAACTCACTGTAGGCACCATCAATAGATCG622450.2884868287652583No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG567550.263042332180452No Hit
CTGTAGGCACCATCAATAGATTGGAAGAGCACACGTCTGAACTCCAGTCA429920.19925497216284013Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGG428900.19878223288202954No Hit
TTGAGTCTTAACCTAATTTTTTGAGGTCCTCTGTAGGCACCATCAATAGA390160.18082740960888935No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG351490.16290502922756953No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT339820.15749633569123636No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCTGTAGGCACCATCAATAGATC338940.15708848219406643No Hit
ATCCTGCCGACTACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC330050.15296823493288375No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGA327230.15166124986240737No Hit
TGAGGGCATCAAGCACATAATATAGCTCAAGACGCCCTGTAGGCACCATC314430.14572883535811737No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA272680.12637896773670276No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT272060.1260916164091512No Hit
AAAAGCTGAACAAAATGAGTGAAAACTCTGTAGGCACCATCAATAGATCG264080.12239312674163293No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA251200.1164236346466911No Hit
CGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCGG243760.11297541871607254No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCTGTAGGCACCATCA235950.10935571892868937No Hit
AAGCTGAACAAAATGAGTGAAAACTCTGTAGGCACCATCAATAGATCGGA233760.10834071988459601No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGAT232350.1076872273493578No Hit
AAGCTGAACAAAATGAGTGAAAACTCCTGTAGGCACCATCAATAGATCGG230360.10676492228189396No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA229080.10617168083146497No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG228220.10577309673195799No Hit
ACCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATTG227480.10543012901842873No Hit
AGGTAAGCATACTGGAAAGTGTGCTTGCTGTAGGCACCATCAATAGATCG225250.10439659117900946No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA223430.10355307599168072No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)