FastQCFastQC Report
Fri 30 Jun 2023
SRR1873533.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1873533.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22648457
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG1827820.8070395259156066No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1774840.78364720386912Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG1361030.6009371852572561No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA1199650.5296828830326057No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1066680.47097248170151285No Hit
TTGAGTCTTAAACCAATTTTTTGAGGCCTCTGTAGGCACCATCAATAGAT960960.4242938050923292No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA948780.4189159552900226No Hit
ACGATTAAAGTCCTACGTGATCTGAGTCTGTAGGCACCATCAATAGATCG884080.3903488877851591No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG796000.3514588212344885No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA755170.33343110305483503No Hit
TCGACTGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAATAGATCGG732390.3233730227185013No Hit
AAGCTGAACAAAATGAGTGAAAACTCACTGTAGGCACCATCAATAGATCG606060.2675943884389122No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG598470.2642431667640758No Hit
ACATATACTAAAATTGGAACGATACAGAGAACTGTAGGCACCATCAATAG591710.2612584159706774No Hit
CTGTAGGCACCATCAATAGATTGGAAGAGCACACGTCTGAACTCCAGTCA462960.20441127622954622Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGG439160.193902834087108No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG400940.1770275122936631No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT381670.16851920640774778No Hit
TTGAGTCTTAACCTAATTTTTTGAGGTCCTCTGTAGGCACCATCAATAGA379610.16760965217188967No Hit
ATCCTGCCGACTACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC376630.16629388924817262No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGA372960.16467346980856135No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCTGTAGGCACCATCAATAGATC332890.14698131532757397No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT300150.1325255844139846No Hit
TGAGGGCATCAAGCACATAATATAGCTCAAGACGCCCTGTAGGCACCATC297090.1311744989956711No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA288390.12733317770830924No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA276550.12210544850803744No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCTGTAGGCACCATCA260890.11519107019078607No Hit
AAAAGCTGAACAAAATGAGTGAAAACTCTGTAGGCACCATCAATAGATCG255720.11290835397749172No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGAT254730.11247123810686087No Hit
CGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCGG248650.10978672851753213No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG247600.10932312077595396No Hit
GCGCAGCGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATAGATCGGAAG233240.10298273299589458No Hit
AAGCTGAACAAAATGAGTGAAAACTCCTGTAGGCACCATCAATAGATCGG226590.10004655063256626No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)