FastQCFastQC Report
Fri 30 Jun 2023
SRR1873534.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1873534.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20210619
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG4132922.044924997101771No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA2361181.1682868298096165No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2173291.07532084989579Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG1566550.7751123307999621No Hit
ACGATTAAAGTCCTACGTGATCTGAGTCTGTAGGCACCATCAATAGATCG1320650.6534436179317417No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG1313880.6500938937100343No Hit
CCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGG1154520.5712442553095479No Hit
TCGACTGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAATAGATCGG1025150.507233350942888No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1013590.5015135855067081No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA818690.4050791319157518No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT743040.36764831398781006No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG668160.33059848389601526No Hit
TTGAGTCTTAAACCAATTTTTTGAGGCCTCTGTAGGCACCATCAATAGAT627170.31031706648866125No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA615430.30450823896091456No Hit
ACATATACTAAAATTGGAACGATACAGAGAACTGTAGGCACCATCAATAG541470.2679136151149057No Hit
AAGCTGAACAAAATGAGTGAAAACTCACTGTAGGCACCATCAATAGATCG487360.24114056081112606No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGA475140.23509423437253454No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT458480.2268510430086283No Hit
CTGTAGGCACCATCAATAGATTGGAAGAGCACACGTCTGAACTCCAGTCA453440.2243573044447575Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG446710.22102737179895382No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA430720.21311568933143513No Hit
GCGCAGCGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATAGATCGGAAG407140.20144855533618242No Hit
TCCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG401060.19844023579881448No Hit
ACCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATTG382580.1892965277312882No Hit
TACCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATC377330.18669888339392277No Hit
AAATATGAACTAAAAAATTTCGGTTGGGCTGTAGGCACCATCAATAGATC349340.17284972815528313No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCTGTAGGCACCATCAATAGATC334340.16542788719138193No Hit
CGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCGG333090.16480940044439016No Hit
ACGATTAAAGTCCTACGTGATCTGAGCTGTAGGCACCATCAATAGATCGG330250.16340419855522484No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATTGGAAGA327650.16211774612148197No Hit
ATCCTGCCGACTACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC323810.16021775483472325No Hit
AAAAGCTGAACAAAATGAGTGAAAACTCTGTAGGCACCATCAATAGATCG316230.1564672512009652No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGAT283820.14043112682496267No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA277680.1373931199237391No Hit
AAGCTGAACAAAATGAGTGAAAACTCTGTAGGCACCATCAATAGATCGGA265430.13133194980321977No Hit
AAGCTGAACAAAATGAGTGAAAACTCCTGTAGGCACCATCAATAGATCGG257190.12725488516705005No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG228250.11293568000069668No Hit
CCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG214580.1061719089355947No Hit
TCGATTAAAGTCCTACGTGATCTGAGTCTGTAGGCACCATCAATAGATCG209330.10357426459822927No Hit
TTGAGTCTTAATCTAATTTTTTGAGGTCCTCTGTAGGCACCATCAATAGA203170.10052636190905385No Hit
CGACTGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAATAGATCGGA202600.1002443319524256No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)