FastQCFastQC Report
Fri 30 Jun 2023
SRR1873535.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1873535.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15608650
Total Bases780.4 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG2569311.646080859010869No Hit
CCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGG1766881.1319877119417758No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA1653391.0592780285290528No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG1306480.8370230609309581No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1030980.6605183664186204Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG900400.5768596259125549No Hit
ACGATTAAAGTCCTACGTGATCTGAGTCTGTAGGCACCATCAATAGATCG838380.53712524785936No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG732390.4692205924279166No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA614680.39380727993772685No Hit
TCCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG569570.36490663830632375No Hit
TCGACTGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAATAGATCGG565300.3621709757089819No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA548420.35135645939911525No Hit
TATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCG524500.3360316234908208No Hit
AAGCTGAACAAAATGAGTGAAAACTCTGTAGGCACCATCAATAGATCGGA447190.28650139505979055No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG430460.2757829793095495No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT413370.26483392221620705No Hit
AAGCTGAACAAAATGAGTGAAAACTCACTGTAGGCACCATCAATAGATCG407890.2613230484378854No Hit
TTGAGTCTTAAACCAATTTTTTGAGGCCTCTGTAGGCACCATCAATAGAT405850.26001608082697736No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT371690.23813078004824248No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA348820.2234786480573272No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG339730.21765495414401628No Hit
ACATATACTAAAATTGGAACGATACAGAGAACTGTAGGCACCATCAATAG337880.21646971390863398No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA331090.21211956190958217No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGA328520.21047303898799702No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA300310.19239972707441066No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG281500.1803487168973614No Hit
TATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCA268490.17201359502583502No Hit
TACCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATC262790.16836177376006253No Hit
ACCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATTG255010.16337735806748183No Hit
CTGTAGGCACCATCAATAGATTGGAAGAGCACACGTCTGAACTCCAGTCA252200.16157707425049572Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCGG245430.15723973565939398No Hit
CCCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG242620.1554394518424079No Hit
AAATATGAACTAAAAAATTTCGGTTGGGCTGTAGGCACCATCAATAGATC241630.154805188148879No Hit
CTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGGA237810.1523578272304139No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGAT235430.15083303168435452No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCTGTAGGCACCATCAATAGATC231070.14803970875123731No Hit
ATCCGAGTCACGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT219460.14060152543621646No Hit
AAGCTGAACAAAATGAGTGAAAACTCCTGTAGGCACCATCAATAGATCGG212870.13637950751666544No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCAGACCGCTGTAGGCACCATCA207630.13302239463374477No Hit
AAAAGCTGAACAAAATGAGTGAAAACTCTGTAGGCACCATCAATAGATCG192270.12318169732808411No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA177890.11396885701197733No Hit
ACGATTAAAGTCCTACGTGATCTGAGCTGTAGGCACCATCAATAGATCGG176550.11311035867932204No Hit
CCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATTGG168610.1080234357231407No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG163010.10443568149711857No Hit
CGACTGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAATAGATCGGA159870.10242397644895619No Hit
ATCCTGCCGACTACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC159860.10241756974498115No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)