FastQCFastQC Report
Fri 30 Jun 2023
SRR1873536.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1873536.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21516655
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG3316381.5413083492764095No Hit
CCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGG2368881.1009517975726246No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA2027940.9424977999600774No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG1857770.8634102280303328No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1578790.7337525279835551Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG1196470.5560669165351213No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1095310.5090521737695752No Hit
ACGATTAAAGTCCTACGTGATCTGAGTCTGTAGGCACCATCAATAGATCG1084980.50425124165443No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA866270.4026044011023089No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA801900.3726880409617573No Hit
TCCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG751920.34945952333204205No Hit
TCGACTGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAATAGATCGG750600.3488460450753149No Hit
TATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCG719050.3341829852270253No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT592840.27552609827131586No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG588210.2733742768102198No Hit
AAGCTGAACAAAATGAGTGAAAACTCTGTAGGCACCATCAATAGATCGGA561160.2608026201098637No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT544940.25326427365220106No Hit
TTGAGTCTTAAACCAATTTTTTGAGGCCTCTGTAGGCACCATCAATAGAT516260.23993506425603792No Hit
AAGCTGAACAAAATGAGTGAAAACTCACTGTAGGCACCATCAATAGATCG510250.23714187916290894No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG500650.23268021911398404No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGA491120.2282510920029159No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA479890.22303187925818393No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA471700.2192255255289449No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA432240.20088624370284322No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG414690.1927297714259024No Hit
ACATATACTAAAATTGGAACGATACAGAGAACTGTAGGCACCATCAATAG410350.19071272927878427No Hit
CTGTAGGCACCATCAATAGATTGGAAGAGCACACGTCTGAACTCCAGTCA356190.16554153050276635Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TACCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATC344700.16020148113170937No Hit
ATCCGAGTCACGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT342630.15923943568365995No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGAT339690.15787305229367668No Hit
TATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCA335460.15590713333461917No Hit
CCCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG327760.1523285101703773No Hit
ACCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATTG324250.15069721571498915No Hit
CGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCGG323770.15047413271254292No Hit
CTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGGA308940.14358179744946414No Hit
AAATATGAACTAAAAAATTTCGGTTGGGCTGTAGGCACCATCAATAGATC299230.13906901421247866No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCTGTAGGCACCATCAATAGATC297120.13808837851422537No Hit
AAGCTGAACAAAATGAGTGAAAACTCCTGTAGGCACCATCAATAGATCGG269360.1251867448727509No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCAGACCGCTGTAGGCACCATCA256540.11922856968241578No Hit
AAAAGCTGAACAAAATGAGTGAAAACTCTGTAGGCACCATCAATAGATCG242550.11272662967361795No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG233460.1085019953147922No Hit
ACGATTAAAGTCCTACGTGATCTGAGCTGTAGGCACCATCAATAGATCGG233150.10835792087571232No Hit
ATCCTGCCGACTACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC230530.10714025948735992No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA223810.10401709745311248No Hit
CCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATTGG220620.10253452499935514No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG216870.10079168904274385No Hit
CGACTGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAATAGATCGGA216790.10075450854233617No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)