FastQCFastQC Report
Fri 30 Jun 2023
SRR1873537.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1873537.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22075170
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2411701.0924944179365323Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG2249101.0188370010287577No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG1887100.8548518539155078No Hit
CCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGG1340670.6073203513268528No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA1306760.5919592012201945No Hit
AGCTTTGCGCAGTGGCAGTATCGTAGCCACTGTAGGCACCATCAATAGAT1210840.5485076672116228No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG881490.3993128931736426No Hit
ACGATTAAAGTCCTACGTGATCTGAGTCTGTAGGCACCATCAATAGATCG800280.3625249545077116No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG793320.35937209090575517No Hit
TCGACTGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAATAGATCGG737510.33409029239638927No Hit
TTTTACTAAAGATTTCCGTGGAGAGGAACACTGTAGGCACCATCAATAGA675470.3059863185651571No Hit
CGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCGG650840.29482898659444073No Hit
TTTTACTAAAGATTTCCGTGGAGAGGAACCTGTAGGCACCATCAATAGAT570850.25859370505414No Hit
CTGTAGGCACCATCAATAGATTGGAAGAGCACACGTCTGAACTCCAGTCA568310.2574430910384835Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG567350.2570082133002826No Hit
AAGCTGAACAAAATGAGTGAAAACTCACTGTAGGCACCATCAATAGATCG554390.2511373638345707No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA544770.24677952649968266No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA543400.2461589197274585No Hit
TACTAAAGATTTCCGTGGAGAGGAACACTGTAGGCACCATCAATAGATCG541030.24508531531127506No Hit
TACTAAAGATTTCCGTGGAGAGGAACCTGTAGGCACCATCAATAGATCGG515610.23357011520183085No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCTGTAGGCACCATCAA509240.2306845202098104No Hit
TTGAGTCTTAAACCAATTTTTTGAGGCCTCTGTAGGCACCATCAATAGAT441210.19986709049126236No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCTGTAGGCACCATCA439160.19893844532114588No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCTGTAGGCACCATCAATAGATC394580.17874381035344236No Hit
ACATATACTAAAATTGGAACGATACAGAGAACTGTAGGCACCATCAATAG392980.17801901412310756No Hit
TTTACTAAAGATTTCCGTGGAGAGGAACACTGTAGGCACCATCAATAGAT375920.1702908743171627No Hit
GCGCAGCGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATAGATCGGAAG363500.16466464357918875No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGA354590.1606284345715118No Hit
CGACTGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAATAGATCGGA345930.15670547497482465No Hit
AGGTAAGCATACTGGAAAGTGTGCTTGCTGTAGGCACCATCAATAGATCG328400.14876442627621894No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT324220.14687089612446927No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT302280.13693212781600322No Hit
TCCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG299810.13581322363542386No Hit
TTACTAAAGATTTCCGTGGAGAGGAACCTGTAGGCACCATCAATAGATCG294900.13358900520358394No Hit
TTTACTAAAGATTTCCGTGGAGAGGAACCTGTAGGCACCATCAATAGATC289210.1310114486094558No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG287280.13013716315661442No Hit
TTACTAAAGATTTCCGTGGAGAGGAACACTGTAGGCACCATCAATAGATC287130.13006921351002054No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT286320.12970228541841355No Hit
TGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTGTAGGCACCATCAATA277620.12576120591596804No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA275980.12501828977987486No Hit
TTTTTACTAAAGATTTCCGTGGAGAGGAACACTGTAGGCACCATCAATAG274980.12456529213591559No Hit
ACTAAAGATTTCCGTGGAGAGGAACACTGTAGGCACCATCAATAGATCGG260550.11802853613358356No Hit
ACCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATTG257110.11647022423836373No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA254560.11531508024626763No Hit
TTCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATC250330.11339890021232No Hit
AAAAGCTGAACAAAATGAGTGAAAACTCTGTAGGCACCATCAATAGATCG240530.10895952330151931No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC240410.10890516358424421No Hit
ACTAAAGATTTCCGTGGAGAGGAACCTGTAGGCACCATCAATAGATCGGA232780.10544879156083509No Hit
TTTTTACTAAAGATTTCCGTGGAGAGGAACCTGTAGGCACCATCAATAGA230150.10425740775722227No Hit
CGCGGCGCGGGAAATGTGGCGTACGCTGTAGGCACCATCAATAGATCGGA226740.1027126857913212No Hit
AAGCTGAACAAAATGAGTGAAAACTCTGTAGGCACCATCAATAGATCGGA224930.10189276005575494No Hit
ATCCTGCCGACTACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC221950.10054282707675638No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGAT221340.10026649851394122No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)