FastQCFastQC Report
Sat 1 Jul 2023
SRR1873538.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1873538.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19998786
Total Bases999.9 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG1962350.9812345609378489No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG1569500.784797637216579No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1194100.5970862431349583No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA938760.4694084930955309No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA938610.46933348854275453No Hit
CCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGG926790.46342312978397787No Hit
ACGATTAAAGTCCTACGTGATCTGAGTCTGTAGGCACCATCAATAGATCG828380.41421514285917155No Hit
TCGACTGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAATAGATCGG652310.32617479881028777No Hit
AGCTTTGCGCAGTGGCAGTATCGTAGCCACTGTAGGCACCATCAATAGAT646910.32347463491033907No Hit
CGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCGG611020.30552854558271686No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA592740.2963879907510386Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTTACTAAAGATTTCCGTGGAGAGGAACACTGTAGGCACCATCAATAGA557490.27876192084859547No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGA522990.2615108737100342No Hit
TACTAAAGATTTCCGTGGAGAGGAACACTGTAGGCACCATCAATAGATCG465910.23296914122687248No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCTGTAGGCACCATCAA461990.2310090222476504No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG461280.23065400069784237No Hit
AAGCTGAACAAAATGAGTGAAAACTCACTGTAGGCACCATCAATAGATCG422340.211182818797101No Hit
TTTTACTAAAGATTTCCGTGGAGAGGAACCTGTAGGCACCATCAATAGAT394990.19750698867421254No Hit
ACATATACTAAAATTGGAACGATACAGAGAACTGTAGGCACCATCAATAG388630.19432679563649513No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGAT382660.19134161443599626No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG365260.18264108631393924No Hit
TTGAGTCTTAAACCAATTTTTTGAGGCCTCTGTAGGCACCATCAATAGAT362470.1812460016322991No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCTGTAGGCACCATCAATAGATC354510.1772657600316339No Hit
CGACTGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAATAGATCGGA353820.1769207390888627No Hit
TACTAAAGATTTCCGTGGAGAGGAACCTGTAGGCACCATCAATAGATCGG353110.17656571753905462No Hit
TTTACTAAAGATTTCCGTGGAGAGGAACACTGTAGGCACCATCAATAGAT344080.17205044346191814No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA341740.17088037243860704No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCTGTAGGCACCATCA338040.16903026013679032No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT320230.16012471957047794No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA314030.1570245313890553No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT306750.15338431042764294No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA256590.12830278797923034No Hit
TTACTAAAGATTTCCGTGGAGAGGAACACTGTAGGCACCATCAATAGATC256470.12824278433700925No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG252700.12635766991056357No Hit
AAAAGCTGAACAAAATGAGTGAAAACTCTGTAGGCACCATCAATAGATCG252180.12609765412760554No Hit
ACCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATTG248720.124367549110231No Hit
TGAGGGCATCAAGCACATAATATAGCTCAAGACGCCCTGTAGGCACCATC247540.123777513295057No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT246070.12304246867784875No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG240770.12039230781308424No Hit
TCCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG239930.11997228231753668No Hit
GCGCAGCGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATAGATCGGAAG233370.11669208320945082No Hit
TGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTGTAGGCACCATCAATA232150.11608204618020314No Hit
TTCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATC231370.1156920225057661No Hit
TTTACTAAAGATTTCCGTGGAGAGGAACCTGTAGGCACCATCAATAGATC229760.11488697363929992No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA219310.10966165646254727No Hit
TTTTTACTAAAGATTTCCGTGGAGAGGAACACTGTAGGCACCATCAATAG207800.10390630711284174No Hit
TTACTAAAGATTTCCGTGGAGAGGAACCTGTAGGCACCATCAATAGATCG207370.10369129406154953No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC207000.10350628283136787No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATTG202420.10121614381992985No Hit
ATCCTGCCGACTACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC201990.10100113076863765No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)