FastQCFastQC Report
Fri 30 Jun 2023
SRR1873539.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1873539.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23180283
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG2166660.9346995461617099No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG1773620.765141650772771No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1519580.6555485107752999No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1168780.5042129986074804No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA1119470.4829406094826366No Hit
CCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGG1056630.4558313632322781No Hit
ACGATTAAAGTCCTACGTGATCTGAGTCTGTAGGCACCATCAATAGATCG916890.39554737101354626No Hit
AGCTTTGCGCAGTGGCAGTATCGTAGCCACTGTAGGCACCATCAATAGAT778030.3356430117785879No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA764770.3299226329549126Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGACTGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAATAGATCGG737340.318089300290251No Hit
CGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCGG701830.30277024659276164No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGA659480.28450040924867054No Hit
TTTTACTAAAGATTTCCGTGGAGAGGAACACTGTAGGCACCATCAATAGA607210.2619510728147711No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG541600.2336468454677624No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCTGTAGGCACCATCAA540840.23331898061814002No Hit
TACTAAAGATTTCCGTGGAGAGGAACACTGTAGGCACCATCAATAGATCG502860.21693436615937778No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGAT468020.20190435121089761No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG455810.19663694356104278No Hit
AAGCTGAACAAAATGAGTGAAAACTCACTGTAGGCACCATCAATAGATCG453780.19576119929165664No Hit
TTTTACTAAAGATTTCCGTGGAGAGGAACCTGTAGGCACCATCAATAGAT433860.18716768902260597No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA414680.17889341558081925No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCTGTAGGCACCATCA413970.17858712078709307No Hit
CGACTGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAATAGATCGGA404990.1747131387481335No Hit
ACATATACTAAAATTGGAACGATACAGAGAACTGTAGGCACCATCAATAG402980.17384602250110578No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT400930.17296165020936113No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCTGTAGGCACCATCAATAGATC390580.16849664863884536No Hit
TACTAAAGATTTCCGTGGAGAGGAACCTGTAGGCACCATCAATAGATCGG385340.1662361067809224No Hit
TTGAGTCTTAAACCAATTTTTTGAGGCCTCTGTAGGCACCATCAATAGAT383560.16546821279101726No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA380370.16409204322483897No Hit
TTTACTAAAGATTTCCGTGGAGAGGAACACTGTAGGCACCATCAATAGAT371610.16031296943182272No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT354550.1529532663600354No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT312460.13479559330660457No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG305710.131883635760616No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG299030.12900187629288218No Hit
GCGCAGCGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATAGATCGGAAG285960.12336346368161252No Hit
TTACTAAAGATTTCCGTGGAGAGGAACACTGTAGGCACCATCAATAGATC281410.12140058859505727No Hit
ACCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATTG271800.11725482385180543No Hit
TCCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG269630.11631868342590986No Hit
AAAAGCTGAACAAAATGAGTGAAAACTCTGTAGGCACCATCAATAGATCG268310.11574923395024989No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA267820.115537847402467No Hit
TTCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATC261250.11270354205770482No Hit
TGAGGGCATCAAGCACATAATATAGCTCAAGACGCCCTGTAGGCACCATC256320.11057673454633837No Hit
ATCCTGCCGACTACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC254340.1097225603328484No Hit
TTTACTAAAGATTTCCGTGGAGAGGAACCTGTAGGCACCATCAATAGATC249860.10778988332454786No Hit
TGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTGTAGGCACCATCAATA245680.10598662665162457No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA235750.10170281355063697No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)