FastQCFastQC Report
Fri 30 Jun 2023
SRR1873540.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1873540.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17475483
Total Bases873.7 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG2250731.2879357898147936No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG1529200.8750544977784019No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1043240.596973485654159No Hit
CCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGG931500.5330324775572727No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA845390.4837577307591441No Hit
TCGACTGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAATAGATCGG676540.3871366531042375No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA675770.3866960358120002No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA667290.3818435232948926Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGATTAAAGTCCTACGTGATCTGAGTCTGTAGGCACCATCAATAGATCG626930.3587483104186591No Hit
TTGAGTCTTAAACCAATTTTTTGAGGCCTCTGTAGGCACCATCAATAGAT615510.35221344096755436No Hit
TTTTACTAAAGATTTCCGTGGAGAGGAACACTGTAGGCACCATCAATAGA599150.3428517540831347No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT501300.2868590241540105No Hit
AAGCTGAACAAAATGAGTGAAAACTCACTGTAGGCACCATCAATAGATCG492540.2818462871669985No Hit
CGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCGG475220.2719352592429062No Hit
AGCTTTGCGCAGTGGCAGTATCGTAGCCACTGTAGGCACCATCAATAGAT442870.25342361066644054No Hit
TACTAAAGATTTCCGTGGAGAGGAACACTGTAGGCACCATCAATAGATCG431260.2467800174679006No Hit
TTTTACTAAAGATTTCCGTGGAGAGGAACCTGTAGGCACCATCAATAGAT411460.23544985852465422No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT399100.2283770926388701No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGA391080.22378780603660567No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG386530.22118415840065767No Hit
ACATATACTAAAATTGGAACGATACAGAGAACTGTAGGCACCATCAATAG375890.21509562854428688No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA367330.21019733760720663No Hit
CTGGATTGAGGCTCCAGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC367010.21001422392731578No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG358050.20488704089037194No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCTGTAGGCACCATCAATAGATC309790.17727120904183308No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGAT303720.1737977714264035No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCTGTAGGCACCATCAA302310.17299092677438443No Hit
TACTAAAGATTTCCGTGGAGAGGAACCTGTAGGCACCATCAATAGATCGG296140.16946026613398896No Hit
TTACTAAAGATTTCCGTGGAGAGGAACACTGTAGGCACCATCAATAGATC292740.16751468328514868No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCTGTAGGCACCATCA290260.1660955522659946No Hit
TCCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG278690.15947484827744102No Hit
TGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTGTAGGCACCATCAATA266760.15264814139901026No Hit
TTTACTAAAGATTTCCGTGGAGAGGAACACTGTAGGCACCATCAATAGAT265460.1519042420744537No Hit
CGACTGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAATAGATCGGA260790.14923192680854658No Hit
TTTTTACTAAAGATTTCCGTGGAGAGGAACACTGTAGGCACCATCAATAG246160.14086019825603674No Hit
TTCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATC238250.1363338569812348No Hit
ACTAAAGATTTCCGTGGAGAGGAACACTGTAGGCACCATCAATAGATCGG236990.13561284686666458No Hit
ACCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATTG234540.13421088275500026No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT231030.13220235457869747No Hit
TTACTAAAGATTTCCGTGGAGAGGAACCTGTAGGCACCATCAATAGATCG223470.12787629389127614No Hit
TGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAATAGATCGG219040.1253413138852872No Hit
TACCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATC213610.1222341036296393No Hit
TCTAAAGATTTCCGTGGAGAGGAACACTGTAGGCACCATCAATAGATCGG197860.1132214771975115No Hit
TGAGGGCATCAAGCACATAATATAGCTCAAGACGCCCTGTAGGCACCATC181340.10376823347314636No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)