FastQCFastQC Report
Fri 30 Jun 2023
SRR1873541.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1873541.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19517126
Total Bases975.8 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG2382241.2205895478668325No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG1645280.842992969354197No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1276850.6542202986238855No Hit
CCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGG1016490.5208195100036758No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1003460.5141433221264238No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA827650.4240634609829336Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA771990.3955449178326768No Hit
TCGACTGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAATAGATCGG735620.37691000201566566No Hit
ACGATTAAAGTCCTACGTGATCTGAGTCTGTAGGCACCATCAATAGATCG662770.33958380962442936No Hit
TTGAGTCTTAAACCAATTTTTTGAGGCCTCTGTAGGCACCATCAATAGAT635430.32557559960416305No Hit
TTTTACTAAAGATTTCCGTGGAGAGGAACACTGTAGGCACCATCAATAGA627060.3212870583507018No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT607130.311075513884575No Hit
CGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCGG518710.2657717124949647No Hit
AGCTTTGCGCAGTGGCAGTATCGTAGCCACTGTAGGCACCATCAATAGAT515660.264208982408578No Hit
AAGCTGAACAAAATGAGTGAAAACTCACTGTAGGCACCATCAATAGATCG509530.26106815112020076No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGA478490.2451641701754654No Hit
TACTAAAGATTTCCGTGGAGAGGAACACTGTAGGCACCATCAATAGATCG448000.22954199301679973No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT435710.2232449593244415No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG434350.2225481354170691No Hit
TTTTACTAAAGATTTCCGTGGAGAGGAACCTGTAGGCACCATCAATAGAT432990.22185131150969667No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA431930.22130819875836225No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG430360.2205037770417632No Hit
CTGGATTGAGGCTCCAGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC397330.20358017876197554No Hit
ACATATACTAAAATTGGAACGATACAGAGAACTGTAGGCACCATCAATAG378620.19399372633040335No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGAT350480.17957561989403564No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCTGTAGGCACCATCAA342680.1755791298370467No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCTGTAGGCACCATCA339320.1738575648894207No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCTGTAGGCACCATCAATAGATC323100.16554691505296426No Hit
TACTAAAGATTTCCGTGGAGAGGAACCTGTAGGCACCATCAATAGATCGG314220.1609970648342384No Hit
TCCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG304960.15625251381786437No Hit
TTACTAAAGATTTCCGTGGAGAGGAACACTGTAGGCACCATCAATAGATC304180.15585286481216548No Hit
CGACTGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAATAGATCGGA282980.14499060978547762No Hit
TTTACTAAAGATTTCCGTGGAGAGGAACACTGTAGGCACCATCAATAGAT276120.1414757480174079No Hit
TGGTTTCTCTTCAGATCGTATAAATCTTTCGCCTGTAGGCACCATCAATA274330.14055860478638094No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT273160.1399591312778326No Hit
TTTTTACTAAAGATTTCCGTGGAGAGGAACACTGTAGGCACCATCAATAG256610.13147939916973433No Hit
TTCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATC254510.13040342107746805No Hit
ACTAAAGATTTCCGTGGAGAGGAACACTGTAGGCACCATCAATAGATCGG245170.12561788041948388No Hit
ACCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATTG243770.1249005616913064No Hit
TGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAATAGATCGG235660.12074523677307818No Hit
TACCTCTAGCATAACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATC228880.11727136464661857No Hit
TTACTAAAGATTTCCGTGGAGAGGAACCTGTAGGCACCATCAATAGATCG227140.11637983994159797No Hit
ATCCGAGTCACGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT216760.11106143394268193No Hit
TCTAAAGATTTCCGTGGAGAGGAACACTGTAGGCACCATCAATAGATCGG210710.10796159229591487No Hit
CTGTAGGCACCATCAATAGATTGGAAGAGCACACGTCTGAACTCCAGTCA197820.10135713629148063Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGATC196350.10060395162689426No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)