FastQCFastQC Report
Fri 30 Jun 2023
SRR1873542.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1873542.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41556106
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA12429992.9911344436362732No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4992721.201440770220386Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG4291161.0326184075091156No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATAG2054210.4943220618409242No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAG1952400.46982265373950105No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCACCAT1538220.37015498997908997No Hit
GTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAGATCGGAAGA1275100.3068381816140328No Hit
GCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCACCAT1221430.293923112045195No Hit
CGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGAAGAGC1117560.2689279885848785No Hit
GTATAGTGGTGAGTATCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA1048430.2522926474391032No Hit
GTGTAATGGTTAGCACTCTGGACTGTAGGCACCATCAATAGATCGGAAGA1020710.24562214756117912No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT1012000.24352618601945045No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAG973540.23427122839661638No Hit
GTATAGTGGTGAGTATCCCCGCTGTAGGCACCATCAATAGATCGGAAGAG973300.23421347515092003No Hit
TAAATGGATTTTTGGAACTAGGAGTCTGTAGGCACCATCAATAGATCGGA904390.2176310744803664No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATAGATCG860840.20715126677172302No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGTAGGCACCATCAATAG844470.20321201413818707No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA831360.200057243092026No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATAG778420.1873178396455144No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC764360.18393446200180547No Hit
GCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC718920.17299984748330366No Hit
GCCCGGATAGCTCAGTCGGTAGAGCACTGTAGGCACCATCAATAGATCGG670840.16142994726214244No Hit
GTATAGTGGTGAGTATCCCCCTGTAGGCACCATCAATAGATCGGAAGAGC658030.15834736777310177No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA654600.15752197763669193No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC648600.15607814649428414No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATAGATCGG620910.1494148657720721No Hit
TTGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG614960.147983066555851No Hit
AAATGGATTTTTGGAACTAGGAGTCTGTAGGCACCATCAATAGATCGGAA601160.14466225492831306No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGCTGTAGGCAC598470.1440149372994669No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAG593070.14271548927129987No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGA574060.13814095093510445No Hit
GTCTAGTGGTTAGGATTCGGCCTGTAGGCACCATCAATAGATCGGAAGAG570140.13719764792206468No Hit
GCCCGGATAGCTCAGTCGGTAGAGCCTGTAGGCACCATCAATAGATCGGA561390.13509206083938663No Hit
TTGGTCTAGGGGTATGATTCTCTGTAGGCACCATCAATAGATCGGAAGAG558430.1343797708091321No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT557720.13420891745728053No Hit
GCGCAGCGGGAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA553860.13328005275566485No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATTGGAAGA553700.1332415505918673No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC532350.12810391811013286No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGA530530.1276659559969358No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA508930.12246816388426769No Hit
TGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCACCA503410.1211398392332525No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGCTGTAGGCAC496120.11938558439522702No Hit
ATTAAATGGATTTTTGGAACTAGGAGTCTGTAGGCACCATCAATAGATCG472800.11377389402173534No Hit
GTCTAGCGGTTAGGATTCCTGGCTGTAGGCACCATCAATAGATCGGAAGA469860.11306641676195552No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG467740.11255626309163809No Hit
GCATTGGTGGTTCAGTGGTAGAATCTGTAGGCACCATCAATAGATCGGAA467060.11239262889549853No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCTGTAGGCACCATCAATAGATCGG465070.11191375823326662No Hit
TTGGTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGG464880.11186803691375703No Hit
TTGGAGTGTGACAATGGTGTTTGTCTGTAGGCACCATCAATAGATCGGAA452860.10897556185846671No Hit
GCGCAGCGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATAGATCGGAAG445790.10727424749566285No Hit
TGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCAT445550.10721649424996653No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATAGA435190.10472347914407573No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGCTGTAGGCACCATCAATAGA430120.10350344182874112No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATA418740.10076497542864098No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)