FastQCFastQC Report
Fri 30 Jun 2023
SRR1873543.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1873543.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42123096
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA10277692.4399179965309292No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC8589432.0391259939677746No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA4099710.9732689164158305No Hit
TTGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG3668130.8708120599682416No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC2690050.6386164017953476No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGC2689750.6385451819590848No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2270680.5390581926836527Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCA2178380.5171462230601473No Hit
TTGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG1930760.45836137020887546No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGCTGTAGGCAC1901000.4512963624516109No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC1704290.40459751581412723No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT1606920.38148193095778143No Hit
TAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC1287410.3056304313434131No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGCTGTAGGCAC1243290.29515636742370505No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1145790.27200992063831203No Hit
TGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG1132140.268769418088357No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCATCA1110110.2635395081121293No Hit
TTGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATC1029090.2443054043321032No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1022350.2427053320107335No Hit
GCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC1008430.23940073160814201No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAG1004420.23844875979676328No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGA974210.2312769222851046No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAG903960.21459961062691119No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCACCAT796320.18904593337583733No Hit
AGCTTTGCGCAGTGGCAGTATCGTAGCCACTGTAGGCACCATCAATAGAT787660.186990054102386No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCAC787390.18692595624974956No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATAG784640.18627310775067435No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGTCCTGTAGGCACCATCAATAGATCG779100.1849579147743556No Hit
GCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA775640.18413651266279193No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC771680.18319641082412366No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCTGTAGGCACCATCAATAGATCGG745750.17704064297648017No Hit
TGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG729510.17318527584012341No Hit
AGGGGGGCGGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCGCTGTAGGCA724890.17208849036167712No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCTGTAGGCACCATCA669800.15901015442929456No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCTGTAGGCACCATCAA603980.14338452235324772No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGAT597900.14194113367165603No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC588440.1396953348348374No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG584070.13865789921994337No Hit
TCGCTTCTGGCGCCAAGCGCCCGTCCGCCTGTAGGCACCATCAATAGATC568500.13496158971790678No Hit
GCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCACCAT562140.1334517291891365No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGC554590.1316593633098574No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCTGTAGGCACCATCAA553910.13149793168099516No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA548940.13031805639357563No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCTGTAGGCACCATCAAT542790.1288580497501893No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATAGATCG512830.1217455621020829No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGA496330.11782847110763178No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGTCCGCCTGTAGGCACCATCAATAGA476000.11300214020355959No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGCCTGTAGGCACCATCAATAGAT470450.11168457323269874No Hit
TTGCGTGCATTTATCAGATCAAAACCACTGTAGGCACCATCAATAGATCG466720.1107990732684986No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA424530.10078319029541419No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)