FastQCFastQC Report
Fri 30 Jun 2023
SRR1873544.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1873544.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56947765
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC13829382.4284324415541154No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA12068942.1193000287193007No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCA7159491.2572029824173083No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA6826241.1986844435422532No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGC6099561.0710797868889148No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC6077341.0671779656321894No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA3175040.557535488881785No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCAC2921670.5130438393850926No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC2562580.4499878090035667No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGC2542990.44654781447524766No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGCTGTAGGCAC2107050.3699969612503669No Hit
TAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC2104180.36949299063799956No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGCTGTAGGCAC2088320.3667079823062415No Hit
TTGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG2031330.35670056586066196No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT1960800.3443155319616143No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1834970.3222198447998793Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG1738760.30532541531700147No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAG1590230.27924361913061907No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATAG1412260.24799217317835034No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAG1392010.2444362829691385No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGA1294410.22729777015831962No Hit
GCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA1288100.22618973720917754No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCACCAT1223080.21477225664606855No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1161330.20392898650192856No Hit
TAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCA1052980.1849027788886886No Hit
GCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCACCAT1007660.17694460880071414No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATAGATCG943000.16559034406354664No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC888100.15594993060746107No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA869970.15276631137323826No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT848140.14893297392794957No Hit
AGGGGGGCGGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCGCTGTAGGCA807090.14172461377544843No Hit
GCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC806170.1415630622202645No Hit
AGCTTTGCGCAGTGGCAGTATCGTAGCCACTGTAGGCACCATCAATAGAT797510.14004237040733733No Hit
TGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG771940.1355522907703226No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGAT756300.13280591433219546No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGA726210.12752212488058137No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCA706030.12397852663752476No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCTGTAGGCACCATCAATAGATCGG690710.12128834204467902No Hit
TTGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATC685420.12035942060237131No Hit
GCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGC681030.11958853872491748No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGTCCTGTAGGCACCATCAATAGATCG676680.11882468082812382No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCTGTAGGCACCATCAA674010.11835583011905734No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCTGTAGGCACCATCAAT656110.11521259877363055No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCATCA655050.11502646328613599No Hit
TTGGTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGG641500.11264708983750284No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCTGTAGGCACCATCA606620.10652217870183317No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGGCTGTAGGC601770.10567052104678734No Hit
TTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA593270.10417792515650087No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATAGATCGG583990.10254836164334107No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG579060.10168265602697489No Hit
GTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAGATCGGAAGA572880.10059745101497837No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)