FastQCFastQC Report
Fri 30 Jun 2023
SRR1873545.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1873545.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46317042
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA12389592.6749527744021306No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA12340152.664278517613452Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC12310472.6578705090882098No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCA9034781.950638384895132No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA8576881.8517762857135824No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA6754601.458340107297871No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGC6625801.4305317684147447No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG6479791.3990077345612872No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC4200960.9070009263544938No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGC3034280.6551109200799136No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCAC2960180.6391124890920279No Hit
GTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAGATCGGAAGA2295900.49569227672181654No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC1973290.4260397285301596No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGCTGTAGGCAC1767160.3815355911545474No Hit
GTGTAATGGTTAGCACTCTGGACTGTAGGCACCATCAATAGATCGGAAGA1756780.3792945153967302No Hit
GCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCACCAT1520550.32829169012995263No Hit
TTGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG1405930.30354485936299647No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATAG1349450.2913506436788429No Hit
GCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC1255500.27106653313482326No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGCTGTAGGCAC1213410.2619791652498016No Hit
GTATAGTGGTGAGTATCCCCGCTGTAGGCACCATCAATAGATCGGAAGAG1123280.24251980512917903No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAG1091930.23575123817276586No Hit
GTCTAGTGGTTAGGATTCGGCCTGTAGGCACCATCAATAGATCGGAAGAG1066060.23016582103839878No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCACCAT994420.2146985120509207No Hit
TAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC969640.20934842946144966No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATAGATCG943040.20360540295297785No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG912780.19707217054146073No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT908240.19609196977648097No Hit
GCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA804180.1736250773527377No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATAG791590.17090685540756254No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGGCTGTAGGC781860.16880611676367416No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA777270.16781512083608446No Hit
TAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCA731540.1579418651130614No Hit
GCGCAGCGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATAGATCGGAAG729890.1575856247469344No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCATCA709970.15328483196314652No Hit
AGGGGGGCGGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCGCTGTAGGCA686380.14819167424379132No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT683700.14761305352790016No Hit
CTGTAGGCACCATTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA678580.14650762887664542Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGC669690.14458824896460357No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCTGTAGGCACCATCAAT654100.14122231726283385No Hit
GTCTAGCGGTTAGGATTCCTGGCTGTAGGCACCATCAATAGATCGGAAGA653610.14111652466925673No Hit
GCGCAGCGGGAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA640080.13819535366701524No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCCTGTAGGCACCATCAA637370.13761025585355818No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG623720.1346631764610529No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGGCTGTAGG612810.13230767197957072No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGGCACCATCAATA606290.13089998277523854No Hit
GTATAGTGGTGAGTATCCCCCTGTAGGCACCATCAATAGATCGGAAGAGC599480.12942968162776888No Hit
TGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG584400.12617386058462024No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCTGTAGGCACCATCAA577770.12474242202254626No Hit
TTGGTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGG572390.12358086252572001No Hit
TGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCAT558540.12059060248277513No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAG558050.12048480988919802No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATA550660.11888928485545341No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT549990.11874462967648063No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAG530390.11451292593339618No Hit
TGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCACCA528100.11401850748586234No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGTAGGCACCATCAATAG524200.11317648480228941No Hit
TTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA523010.11291955993217356No Hit
TTCCACCTTTCGGGCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCA518000.11183788463866064No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCTGTAGGCACCATCA504750.10897716654703468No Hit
AGCTTTGCGCAGTGGCAGTATCGTAGCCACTGTAGGCACCATCAATAGAT498210.10756515927765854No Hit
TTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCACC496290.10715062503343803No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC473690.102271211533759No Hit
CTGTAGGCACTATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA467030.10083329587411909Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)