FastQCFastQC Report
Fri 30 Jun 2023
SRR1873546.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1873546.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52710370
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA32692766.202339311979787No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGC27218125.163712567375263No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA15000372.845810037000309No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC9251071.7550758987273283No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCA8155411.5472116777021296No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGC6015701.1412744778683968No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGCTGTAGGCAC3507170.6653662267974974No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC3276270.6215608048283477No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCACCAT3206680.6083584691209718No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCAC3012850.5715858188815598No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGGCTGTAGG2997090.5685958948874765No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGCTGTAGGCAC2310770.4383900169928612No Hit
TAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC2155140.4088645175512902No Hit
TAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCA2131080.40429995084458714No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGC1938290.3677246052342262No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATAGATCG1749840.331972627018175No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT1745910.33122704317954893No Hit
TTGGTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGG1291810.24507701236018642No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1270020.24094310094958543Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA1188980.22556851716275186No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAG1163510.22073645091089283No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC1093030.20736526797288657No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA1039260.19716423921896203No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATAG1022560.19399598219477496No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1006420.19093396612469235No Hit
TTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCACC1005820.190820136531009No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAG911280.1728843868862996No Hit
GCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA886490.16818132750728176No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGGCTGTAGGC885630.16801817175633563No Hit
GCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCACCAT848170.16091141079070398No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAG777720.14754591933238184No Hit
GCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGC759020.14399823032925022No Hit
ACAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA752940.14284475711325875No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT751800.14262848088526034No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCTGTAGGCACCATCAA719670.13653290614351596No Hit
TTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA681760.1293407729826218No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATA680500.12910173083588675No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGGCTGTAGGCACCA679890.12898600408230865No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCTGTAGGCACCATCA661520.12550092135570287No Hit
ACAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGC637680.12097809216668372No Hit
TTGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG616800.11701682230650248No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCTGTAGGCACCATCAATAGATCGG611530.11601701904198358No Hit
TCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCACCATCAATAGATCGGA604510.11468521279588817No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGG601090.11403638411189296No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGA579150.10987401530287114No Hit
TTTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATAGATC563690.1069410061056297No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATAG538870.1022322552469277No Hit
TTGGTCTAGGGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAA536080.10170294763630003No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)