FastQCFastQC Report
Fri 30 Jun 2023
SRR1873547.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1873547.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54709618
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA27694665.062119059211856Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA9109591.6650801692674952No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA5136990.9389555598798003No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCACCAT3551510.6491564243786165No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC3544560.6478860810177838No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG3438160.6284379466879114No Hit
TTGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG2949230.5390697482113657No Hit
GCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCACCAT2761010.5046662910349694No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGCTGTAGGCAC2288490.41829756515572825No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT2226650.4069942509925768No Hit
GCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC2063010.3770836052995289No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGCTGTAGGCAC2029330.37092746653796776No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC1903050.34784560184646146No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC1877270.3431334505022499No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCATCA1791450.32744699478618183No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAG1723110.31495558971002136No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCCTGTAGGCACCATCAA1585970.2898886992777029No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATAG1512160.27639747000244086No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAG1479460.2704204587939181No Hit
CTGTAGGCACCATTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1380900.25240534488835215Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT1342580.24540109199811996No Hit
GTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAGATCGGAAGA1175080.21478490308596193No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGC1134420.20735293746704644No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATA1118450.20443388948539176No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA1059330.1936277456735304No Hit
TTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCACC1058890.19354732105788053No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATAGATCG1053960.19264619979616746No Hit
GTGTAATGGTTAGCACTCTGGACTGTAGGCACCATCAATAGATCGGAAGA1052830.19243965476052124No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATAG1047010.19137585643533464No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1016580.18581376313027811No Hit
TTGGGGGGGACGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATC1013000.18515939921203617No Hit
CTGTAGGCACTATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1010700.18473899781204833Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGGCACCATCAATA970590.17740756296269514No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA968840.17708769233226962No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCCTGTAGGCACCATCAAT966920.17673674855488847No Hit
AGCTTTGCGCAGTGGCAGTATCGTAGCCACTGTAGGCACCATCAATAGAT951840.17398037763670732No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT949190.17349600211063437No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC942220.17222200308545382No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA929380.16987506657421736No Hit
CTGTAGGCATCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA907010.16578620600129212Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGGTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGG902060.16488142907523134No Hit
TTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA895630.16370613298743925No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGGCACCATCAAT873130.15959351059625385No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC846570.15473878834248123No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCTGTAGGCACCATCA834730.15257463504863075No Hit
GTATAGTGGTGAGTATCCCCGCTGTAGGCACCATCAATAGATCGGAAGAG826000.15097893756085082No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGA824300.15066820609129458No Hit
CTGTAGGTACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA822520.15034285196434746Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTAGGCACCATCAATAGATTGGAAGAGCACACGTCTGAACTCCAGTCA808110.14770894580181496Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCA790470.1444846498471256No Hit
TGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCACCA774650.1415930193480788No Hit
TGGGGGGGACGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG771290.14097886773766177No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCTGTAGGCACCATCAA767140.1402203173855098No Hit
TGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCAT750290.1371404201725554No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGCAC743010.13580975834998518No Hit
GCGCAGCGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATAGATCGGAAG704140.12870497469019068No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA703470.12858250993454204Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCCGGATAGCTCAGTCGGTAGAGCACTGTAGGCACCATCAATAGATCGG676050.12357059411381742No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGGGCTGTAGGCACCA675810.1235267261416448No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCTGTAGGCACCATCA674380.12326534614078277No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAG645460.11797925549397914No Hit
TAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC638790.11676009143401439No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGTAGGCACCATCAATAG612780.11200589994980407No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCCTGTAGGCACCATCAA593570.10849463434381867No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCCTGTAGGCACCATCAAT567470.1037239923700436No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGTCCGCCTGTAGGCACCATCAATAGA567290.1036910913909141No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG565940.103444334047443No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)