FastQCFastQC Report
Sat 23 Sept 2023
SRR18884275_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18884275_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63115399
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length85
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG12194271.932059401224731No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG9314751.4758284265936432TruSeq Adapter, Index 7 (100% over 49bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT5444040.8625533683150763No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCAGATCGGAAGAGCACACGTCTG5394250.8546646437266443Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC5279790.8365296082498028No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCAGATCGGAAGAGCACACGTCTGA3866890.612669817709621Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT3736820.5920615347769567Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTG3049440.48315308915340927Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC2919170.46251311823284197No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA2866170.4541158014385681Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC2808620.4449975829195027No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT2807890.4448819217636571Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC2804870.44440343314632297No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT2657510.42105572365945115Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG2553820.40462708633118205No Hit
TGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTGA2062690.32681247883737535Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTC2017550.3196604999676862Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGAAGATCGGAAGAGCACACGT2009190.3183359420733441No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACG1843620.29210304128791137No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC1838050.291220530824815No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1793390.2841446031260929No Hit
ATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGA1701850.26964101106292615Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG1644570.2605655713275297Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCT1618470.2564302889061986Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTG1610500.25516752258826725Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG1608510.2548522271086332No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTC1601520.2537447319314261No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA1577230.2498962258006164No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCAGATCGGAAGAGCACACGTCTGA1557550.24677812779096905Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC1526180.24180786688839598No Hit
TGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGAA1489440.23598678351062946Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA1357820.21513291867171752No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTCT1354270.21457045688644066Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC1340380.21236972612658284No Hit
CGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG1294800.20514803368350726Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCAC1271750.20149599307769567No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG1246050.1974240866321704Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC1213600.19228271059492152No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGT1210210.19174559919996703No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACA1172120.18571062190385582No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC1130130.17905772884363766No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG1079650.17105968069694052No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTG1072410.1699125755348548Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT1052020.16668198516815205No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTG1013400.16056303470409813Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA1001400.1586617554299229No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA999400.1583448755508937No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA988240.15657668582591072No Hit
GGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGT985800.1561900923734951No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCT957840.15176011166466682Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACAC956280.15151294535902401No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGT916080.145143659790537No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGA910200.14421203294619117Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGTC903010.14307284978108115No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGAGATCGGAAGAGCACA897820.14225054649500038No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATGTCGTATG897500.1421998457143557TruSeq Adapter, Index 7 (97% over 49bp)
CGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCTGAACT894440.14171501949944101Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGT885600.14031441043413193No Hit
CGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA881450.13965688468514634Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTGGTATG880600.1395222107365589TruSeq Adapter, Index 7 (97% over 49bp)
GGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCTG859170.13612684283276097Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGAGGTGCGTGGCGGGGGCGGGCGGGAGATCGGAAGAGCACACGTCTGAA850540.13475950615474996Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT831650.13176657569731914No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC825790.13083811765176356No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA823110.13041349861386442No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGT819970.12991599720378857No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCAGATCGGAAGAGCACAC819880.12990173760923227No Hit
GGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTG806910.12784677159372787Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCA791250.12536560214092918No Hit
GGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGT786340.1245876620379125No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACG766500.12144421363794278No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAGATCGGAAGAGCA758640.120198875713358No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA730860.11579741419364235No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTG730720.11577523260211031Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCAC724440.11478022978195859No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC715470.11335902352451262No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT711610.11274744535798625Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTC701390.11112818917614702No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGT700290.11095390524268095No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG688900.10914927433160963Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTG688200.1090383663739494Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT673760.10675049364735854Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT671330.10636548459433808Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGA666010.10552258411612038Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACG661870.10486664276652992No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTAGATCGGAAGAGCACACGT653290.10350722808549465No Hit
ATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGTCTGAA645540.10227931855425647Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTGA640600.10149662525305433Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGTCT634710.10056341400931333Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC631320.10002630261435881No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)