FastQCFastQC Report
Sat 23 Sept 2023
SRR18884276_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18884276_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57394469
Total Bases4.8 Gbp
Sequences flagged as poor quality0
Sequence length85
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG16493292.873672374249163No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG16363302.851023850399243TruSeq Adapter, Index 6 (100% over 49bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT12445402.1683970976367077No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCAGATCGGAAGAGCACACGTCTG6320861.1013012421109776Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCAGATCGGAAGAGCACACGTCTGA4707020.8201173531198624Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC3746700.6527980945341615No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC3196790.5569857262726832No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA2534030.44151118464045724Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT2471450.43060769496795936Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT2267730.3951129855387285Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC2201630.3835961963512547No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC2188540.381315488779938No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCAC2152480.37503265340777No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGAAGATCGGAAGAGCACACGT2083030.36293218428416857No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA1955190.3406582609902707No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTG1857850.3236984386073857Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGT1776760.3095698994967616No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC1772010.30874229361717764No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1762930.3071602596410466No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA1637190.28525222526233324No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTAGATCGGAAGAGCACACGTCTG1581720.27558753091696Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1557940.27144427453453746Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG1520920.26499417565828515No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG1514710.26391218986623954No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACG1454480.2534181473131148No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCAC1445530.25185876360316184No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA1389330.24206687930155782No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC1384830.24128283162616246No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCAGATCGGAAGAGCACACGTCTGA1315060.22912660800119955Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTC1252990.2183119770652465Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA1237270.21557303718586543No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC1220760.2126964533812483No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC1213480.21142803847527536No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTC1180710.20571842907022975No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC1171460.20410677551525044No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC1115860.19441943090369912No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACA1107260.19292102867961022No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCT1101560.19192790162410944Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTCT1085170.18907222575750288Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC1062910.18519380325654725No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG1043070.18173702417213755No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG1038540.18094774951223958Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG1025490.17867401125359308Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC1020310.17777148526280467No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTAGATCGGAAGAGCACACGTCTGAA1010570.17607445762761564Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACA960700.16738546705606772No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA946590.16492704201166145No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACACG933390.16262716883050177No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGAGATCGGAAGAGCAC928290.1617385814650537No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGT922430.16071757715887225No Hit
CGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCTGAACT865570.1508106991982102Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACGT839060.14619178722604786No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGC834760.1454425861140034No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTG828420.14433795005577976Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC826200.14395115320258473No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTG797030.13886878193785537Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG795430.13859000943104813Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACG750930.130836649085472No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTG749400.13057007287583755Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCCGCCCCTTGCCTCTCGGCGCCCAGATCGGAAGAGCACACGTCTGAAC747590.1302547114775119Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCCGCCCCTTGCCTCTCGGCGCCCCAGATCGGAAGAGCACACGTCTGAA734510.12797574623436275Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG713230.12426807189382656No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCAGATCGGAAGAGCACACGTCTGAA693520.12083394307559497Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCA680030.1184835423775765No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGAGATCGGAAGAGCACACGTCTGAA664830.11583520356290777Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA648120.11292377319493975No Hit
CGGGGGGGGATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACT638680.11127901540477707Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT635390.1107057894376547Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACAC632820.11025801109859558No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGAGATCGGAAGAG628680.10953668723723187No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGA623670.10866378082529171Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCAGATCGGAAGAGCACACGTCTGAA619110.10786927918089109Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGA608680.10605203090214145Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT606720.1057105345813026No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCTG586970.10226943645040082Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT581940.10139304538212558Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCAGATCGGAAGAGCACAC580530.1011473771105017No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAGATCGGAAGAGCACACG577170.10056195484620652No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT575940.10034764848159845No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAGATCGGAAGAGCACA574190.10004274105227806No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)