FastQCFastQC Report
Sat 23 Sept 2023
SRR18884277_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18884277_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60353272
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length85
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG9892791.6391472528614521No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT8639731.4315263636410633No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCAGATCGGAAGAGCACACGTCTG7082931.1735784598389298Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCAGATCGGAAGAGCACACGTCTGA5607490.92911118389737Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGAAGATCGGAAGAGCACACGT4341000.719265063209829No Hit
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTC4110800.6811229720900633Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTG3979600.6593842998271908Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA3908560.6476136041141233Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT3709080.6145615435729814Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC3360370.5567834002438178No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG3148850.5217364188639184TruSeq Adapter, Index 5 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATG3107860.5149447406927664TruSeq Adapter, Index 5 (97% over 49bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC2789850.46225331412023524No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT2699690.44731460458349304Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA2670220.4424316878793249No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACG2212530.36659652851961366No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC2192110.363213116266505No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG2074410.34371127384775424Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGAA2071320.3431992883500997Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC1978460.32781321284453313No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA1928790.31958333592916055No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC1848290.3062452024142121No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGA1805260.2991155143999483Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTG1723870.2856299158063874Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGA1711440.28357037543879976Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC1695080.28085966904992327No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1600560.2651985463190794No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACA1581890.2621050934902088No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCT1572780.2605956475731755Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG1550430.2568924514979072No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTC1531280.2537194669412456No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1523160.25237405521278117No Hit
TGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTGA1523000.25234754463685083Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCTGAACT1506770.2496583780909178Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1486950.2463743804975478Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG1435920.23791916368676747Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT1423170.23580660216731913No Hit
ATGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTG1403730.23258556719178372Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTCT1391940.2306320691279174Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC1361910.22565636540799314No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC1350640.22378902671590034No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGT1260000.20877078545136707No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGA1190320.19722542963370734Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTG1165510.1931146334535102Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCTG1161550.19245849669923448Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACG1153350.19109982968280495No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC1127510.18681837167005627No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC1112740.18437111412948748No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG1101210.1824606957515079No Hit
ATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGTCTGAA1083740.17956607224211474Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGAGGTGCGTGGCGGGGGCGGGCGGGCAGATCGGAAGAGCACACGTCTGA1069040.17713041307851543Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA1066940.17678246176942983No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTGA1066620.17672944061756915Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCAGATCGGAAGAGCACAC1062860.17610644208320636No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTG1048990.17380830653224566Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG1024850.1698085233887568No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACGT1015000.1681764660580457No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1009670.1672933324973665No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCT976030.16171948390801413Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA963660.15966988500640034No Hit
GGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACAC953850.15804445531967182No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG944960.15657146144454273Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA942100.15609758489978803Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGAGACCGGGGTTCAATTCCCCGACGGGAGATCGGAAGAGCACACGTCT937330.155307238354865Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGTC919640.15237616280356764No Hit
GGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTCTGA900250.1491634123830105Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTAGATCGGAAGAGCACACGTC898960.14894967086457217No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA895910.14844431301090022No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT882780.14626878887361733Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTG866630.1435928776156494Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGT846790.1403055662002882No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC846700.1402906540013274No Hit
CGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT846560.14026745724738834Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCC840760.13930644886991378Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGT828910.13734300934007357No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG804350.13327363593476754Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA801750.13284283907589967No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTAGATCGGAAGAGCACACGT793730.13151399645739173No Hit
GTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGAACTCC785760.13019343839386205Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTGAACT743130.12313002681942414Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG739740.12256833399190022No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC721090.11947819498502087No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAGATCGGAAGAGCA709410.11754292294210661No Hit
CCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC697570.11558114032326201Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTGA696350.11537899718179323Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACG664550.11011002021563968No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCA662240.10972727377564549No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGT651480.10794443754433065No Hit
GATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTGAAC648340.10742416749169788Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACG648260.10741091220373272No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT647660.10731149754399398No Hit
CCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACT646560.10712923733447294Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCAC635160.10524035879943676No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGAGATCGGAAGAGCACACGTCTGAA634290.10509620754281558Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCTGAACT633000.10488246602437727Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGTCTGAAC630200.10441853094559646Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCAC626180.10375245272534685No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCAGATCGGAAGAGCACACGTCTGAAC623060.10323549649470537Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCAGATCGGAAGAGCACACG619820.10269865733211615No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGT611470.10131513665075194No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGAGATCGGAAGAGCACACGTC603860.10005422738306549No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)