FastQCFastQC Report
Thu 21 Sept 2023
SRR18884278_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18884278_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38289407
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length85
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG14774523.8586442459137587No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG13135483.4305780708486813TruSeq Adapter, Index 3 (100% over 49bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA10326532.6969678584993497No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC5886041.5372502373828878No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT5451221.423688802493076Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC4486381.171702659171504No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA3483120.9096824090276456No Hit
AGAGCTAATACATGCCGACGGGCGCTGACCAGATCGGAAGAGCACACGTC2893520.7556972611249895No Hit
AGAGCTAATACATGCCGACGGGCGCTGACAGATCGGAAGAGCACACGTCT2839680.7416359307941228Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC2429270.6344496272820312No Hit
AGGGGCTCGTCCGAAAGCCGCCGTGGCGCAATAGATCGGAAGAGCACACG1938020.5061504347664616No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC1927570.5034212203913213No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC1648820.4306204063176011No Hit
GTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCA1556720.4065667561788043No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA1503520.39267257390536237No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC1386530.36211843134577665No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAGATCGGAAGAGCAC1366050.356769693508181No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGAGATCGGAAGAGCACACGTCT1313370.34301131903139687Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACACG1210710.3161997259450897No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA1148000.2998218280058503No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA1100410.28739280292327324No Hit
AGAGCTAATACATGCCGACGGGCGCTGAAGATCGGAAGAGCACACGTCTG1074070.2805136156848812Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCAGATCGGAAGAGCAC1014710.26501063335872505No Hit
ATCTAAGGCTAAATACCGGCACGAGACCGATAGATCGGAAGAGCACACGT990800.258766086400868No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTC989860.25852058769152525No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA938270.2450468872500428No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT884560.23101950886834055No Hit
GTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCAC859310.22442499566525018No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT856200.22361276057370122Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGCCGAACCACCTCACCCCGAGTGTTACAGATCGGAAGAGCACACGTC844640.22059364878646462No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGT822090.21470429145063544No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCA783200.20454743527367766No Hit
GCTAATACATGCCGACGGGCGCTGACCAGATCGGAAGAGCACACGTCTGA732850.1913975841934559Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACAC716260.18706479314239574No Hit
GCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGAGATCGGAAGAGCACAC710090.18545338140128417No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG702300.1834188761398159Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCAC687780.1796267045869893No Hit
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTAGATCGGAAGAGCACACGT638270.16669623533213768No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG596250.15572192068683643Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACGT581530.15187751536606456No Hit
GCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACA555970.14520203982265903No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC550800.1438517969212738No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGT547890.14309179559767013No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACA533490.1393309643056107No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCGGAAGAGCACACG486590.12708214572244486No Hit
GCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGC473960.12378358327670104No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC461570.12054770135249157No Hit
GTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGAGATCGGAAGAGCACACGT461220.12045629225858734No Hit
TTAAATCAGTTATGGTTCCTTTGGTCGCTCGAGATCGGAAGAGCACACGT450260.11759388177518654No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG445270.11629064926495206No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCAGATCGGAAGAGCACA442950.11568473755678693No Hit
ACTCTGGACTCTGAATCCAGCGATCCGAGTTCAGATCGGAAGAGCACACG434190.11339689852078408No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGAGATCGGAAGAGC423610.11063373219647929No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGT417820.10912156461446373No Hit
GCTAATACATGCCGACGGGCGCTGACAGATCGGAAGAGCACACGTCTGAA412510.10773475807551681Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACACGTC408060.10657255673873454No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGT405340.10586217749467888No Hit
AGAGCTAATACATGCCGACGGGCGCTGACCCAGATCGGAAGAGCACACGT395330.10324787740901811No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACG388200.10138574358176924No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAGATCGGAAGAGCA383100.10005378249916484No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)