FastQCFastQC Report
Sat 23 Sept 2023
SRR18884279_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18884279_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40922817
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length85
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG22065715.392031052016776TruSeq Adapter, Index 2 (100% over 49bp)
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG19289024.713512268717963No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA15446153.774459123867255No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC7053451.7235983534564594No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT5557591.358066332530334Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGGCTCGTCCGAAAGCCGCCGTGGCGCAATAGATCGGAAGAGCACACG4944451.2082379372856955No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC4190491.023998421223055No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA4036390.986342167011621No Hit
AGAGCTAATACATGCCGACGGGCGCTGACCAGATCGGAAGAGCACACGTC3734820.9126497816609254No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAGATCGGAAGAGCAC3644610.8906058446562953No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA3603300.8805112316681425No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC3424770.8368852026975562No Hit
AGAGCTAATACATGCCGACGGGCGCTGACAGATCGGAAGAGCACACGTCT2936360.7175361363808361Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCA2924220.7145695761853345No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC2557110.6248616755781988No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC2050840.5011482958272399No Hit
ATCTAAGGCTAAATACCGGCACGAGACCGATAGATCGGAAGAGCACACGT2013870.49211421589085613No Hit
GTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCAC2002980.4894531087632604No Hit
GCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCAC1906730.4659332225345093No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA1730540.4228790017070428No Hit
ACTCTGGACTCTGAATCCAGCGATCCGAGTTCAGATCGGAAGAGCACACG1708000.4173710719865643No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCA1601290.3912951544855771No Hit
CGCGCCGAACCACCTCACCCCGAGTGTTACAGATCGGAAGAGCACACGTC1492020.3645936691015186No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCAGATCGGAAGAGCAC1420050.3470069032637709No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGAGATCGGAAGAGCACAC1329720.324933642764622No Hit
CGATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACACGTC1316160.3216200878839792No Hit
GTCGATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACACG1253140.3062203660124375No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA1207300.2950187911061939No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGT1202180.2937676553400515No Hit
GGTACAGTGAAACTGCGAATGGCTCATTAGATCGGAAGAGCACACGTCTG1149150.2808091143872134Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACTTTCGATGGTAGTCGCCGTGCCTACCAGATCGGAAGAGCACACGTCT1128830.27584366931533577Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACACG1111390.2715819881119132No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACAC1086980.2656171006018476No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC1023360.25007076125771105No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGAGATCGGAAGAGCACACGTCT1022840.24994369278146222Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGCTAATACATGCCGACGGGCGCTGACCCAGATCGGAAGAGCACACGT997770.24381752605154233No Hit
GCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGC995970.2433776736337579No Hit
CGCGCCGAACCACCTCACCCCGAGTGTTAGATCGGAAGAGCACACGTCTG983720.2403842335682805Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACA968880.23675789474610215No Hit
GATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACACGTCT961840.23503758306765635Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCAC958070.2341163366148523No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACA953720.2330533599385399No Hit
AGAGCTAATACATGCCGACGGGCGCTGAAGATCGGAAGAGCACACGTCTG927160.22656309315167625Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGCTAAATACCGGCACGAGACCGATAGATCGGAAGAGCACACGTCTGA913700.22327397451646597Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT899100.21970628268332557Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTCTGA894430.21856511002162926Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTAATACATGCCGACGGGCGCTGACCAGATCGGAAGAGCACACGTCTGA887940.21697919769306206Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTAGATCGGAAGAGCACACGT883950.21600419150030653No Hit
GTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCACACGT870590.21273950911052872No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGAGATCGGAAGAGC858520.20979005428682979No Hit
CGGTACAGTGAAACTGCGAATGGCTCATTAGATCGGAAGAGCACACGTCT858060.2096776475578404Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACACGTC835950.2042747936927216No Hit
CGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACGT817110.1996710050532445No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC815880.19937043923442516No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA776650.18978410015126768No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC720490.17606070471639332No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCAGATCGGAAGAGCACACGTCTG716470.17507836765000806Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGT699550.17094375492283437No Hit
CGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCACACG698110.17059187298860684No Hit
AGCGGTGGATCACTCGGCTCGTGCGTCGATAGATCGGAAGAGCACACGTC696290.1701471333217359No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT673200.16450480425137887Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACACG632340.1545201543676722No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG622110.15202032645993066No Hit
ACCATGGTGACCACGGGTGACGGGGAATCAGATCGGAAGAGCACACGTCT617970.15100866589902645Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG614090.15006053957624668Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAACCACCTCACCCCGAGTGTTACAGATCGGAAGAGCACACGTCTG603140.14738477070139136Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTAGATCGGAAGAGCACACGTC594630.14530524621508828No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCGGAAGAGCACACG560610.13699203551896244No Hit
AAGAGGGCGTGAAACCGTTAAGAGGTAGATCGGAAGAGCACACGTCTGAA543320.13276700868368863Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGAGATCGGAAGAG525170.12833183013769556No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG522140.12759141190109177Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT515290.12591752908896764No Hit
CGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCCCACAGATCGGAAGAGCA505410.12350322804023975No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAGATCGGAAGAGCA499050.12194908283073476No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACG474130.11585957046896356No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA460040.11241650348752873No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC456590.11157345302010857No Hit
GTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGC446760.10917137009409689No Hit
AAGCCATGCATGTCTAAGTACGCACGGCAGATCGGAAGAGCACACGTCTG440770.107707638992692Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCCCGACCCGGGGAGGTAGTGACGAAAAATAGATCGGAAGAGCACACG435010.10630011125578184No Hit
GCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGAGATCGGAAGAGCACAC420470.10274708116990089No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACG416330.1017354206089967No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)