FastQCFastQC Report
Thu 21 Sept 2023
SRR18884280_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18884280_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37031648
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length85
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG23675686.3933638600150875No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA11458393.094215520735129No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA8721372.355112578300593No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC7963482.1504524994404783No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG7231531.952797239809581TruSeq Adapter, Index 1 (100% over 49bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT4169431.1259099243976396Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGGCTCGTCCGAAAGCCGCCGTGGCGCAATAGATCGGAAGAGCACACG3743921.0110055053450497No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC3470160.9370795488226719No Hit
AGAGCTAATACATGCCGACGGGCGCTGACCAGATCGGAAGAGCACACGTC3101500.8375268635087479No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC3017550.8148570649623803No Hit
AGAGCTAATACATGCCGACGGGCGCTGACAGATCGGAAGAGCACACGTCT2901320.7834703980768017Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA2349020.6343276972172559No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAGATCGGAAGAGCAC2341540.6323078033146134No Hit
GTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCA2226880.6013450981171564No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC2152660.5813027818799746No Hit
AGAGCTAATACATGCCGACGGGCGCTGAAGATCGGAAGAGCACACGTCTG1617050.43666703680052266Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC1569600.4238536724047496No Hit
GTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCAC1414030.3818436597798726No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGAGATCGGAAGAGCACACGTCT1349300.36436401642184546Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA1345250.3632703572900671No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA1268130.34244492710667374No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGAGATCGGAAGAGCACAC1218690.32909418452022443No Hit
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTAGATCGGAAGAGCACACGT1153480.31148492230213465No Hit
GCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCAC1143310.308738622704558No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC1125850.3040237366697804No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACACG1059510.2861093300519599No Hit
ACTCTGGACTCTGAATCCAGCGATCCGAGTTCAGATCGGAAGAGCACACG993870.26838395093839734No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT978170.2641443340571827No Hit
ATCTAAGGCTAAATACCGGCACGAGACCGATAGATCGGAAGAGCACACGT969780.26187870439900485No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCGGAAGAGCACACG958320.25878405411501No Hit
GCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGAGATCGGAAGAGCACAC896890.24219554041991326No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCAGATCGGAAGAGCACA892990.2411423871819045No Hit
CGATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACACGTC876270.23662733022305676No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCAGATCGGAAGAGCAC874920.23626277717913066No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCA841060.2271192467588804No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA800530.21617455426234336No Hit
CGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACGT794650.21458672322657635No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC789720.2132554295180166No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTC787820.21274235486360205No Hit
GTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGAGATCGGAAGAGCACACGT775100.2093074550719428No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG750180.20257807592035873No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACAC746860.20168154547159228No Hit
GTCGATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACACG733830.1981629334994759No Hit
GATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACACGTCT723650.19541393350898129Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGC719480.19428786966218733No Hit
GTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCACACGT716960.1936073706468586No Hit
AGAGCTAATACATGCCGACGGGCGCTGAGATCGGAAGAGCACACGTCTGA680910.18387245417757267Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCAGATCGGAAGAGCACA633690.17112119881891294No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACG632200.17071884027413525No Hit
ACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACAGATCGGAAGAGCACAC618760.16708951219238205No Hit
AGAGCTAATACATGCCGACGGGCGCTGACCCAGATCGGAAGAGCACACGT609260.16452413892030945No Hit
CGCGCCGAACCACCTCACCCCGAGTGTTACAGATCGGAAGAGCACACGTC603520.16297411338539403No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT593340.16022511339489942No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTCTGA587560.15866428628831208Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACA585750.15817551517015932No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGT585240.1580377951313428No Hit
GGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGT577640.15598549651368473No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC575390.15537790810714122No Hit
GGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTAGATCGGAAGAGCACAC511520.13813049854005957No Hit
ACCATGGTGACCACGGGTGACGGGGAATCAGATCGGAAGAGCACACGTCT494350.13349392389990314Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT493060.13314557321348486Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGCTAAATACCGGCACGAGACCGATAGATCGGAAGAGCACACGTCTGA486330.1313282087796903Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTACAGTGAAACTGCGAATGGCTCATTAGATCGGAAGAGCACACGTCTGA475660.12844688953621508Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG473640.1279014101667849Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTAATACATGCCGACGGGCGCTGACCAGATCGGAAGAGCACACGTCTGA470860.12715070093558895Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCAC470560.12706968914804978No Hit
AAACGGGTGGGGTCCGCGCAGTCCGCCCGGAGGATTCAGATCGGAAGAGC466500.12597332962335353No Hit
GGGACGGCCGGGAAGGCCCGGTGGGGAAGGTAGATCGGAAGAGCACACGT446130.12047262924944631No Hit
GGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCAGATCGGAAGAGCACA443090.11965170980238309No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACG431920.11663537091300934No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG431710.11657866266173193Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACTTTCGATGGTAGTCGCCGTGCCTACCAGATCGGAAGAGCACACGTCT423860.11445885422112459Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGGCTCGTCCGAAAGCCGCCGTGGCGCAGATCGGAAGAGCACACGTCT417860.1128386184703419Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCCCACAGATCGGAAGAGCA410240.11078091906684791No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACAC399390.10785099275084922No Hit
AGCGGTGGATCACTCGGCTCGTGCGTCGATAGATCGGAAGAGCACACGTC397820.10742703106272776No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGT389460.10516950258330389No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC384210.10375179630136905No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACACGTC379030.10235299276985999No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACG370610.10007926193292829No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)