FastQCFastQC Report
Thu 21 Sept 2023
SRR18884287_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18884287_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24134677
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length85
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG12099615.013371424030245TruSeq Adapter, Index 9 (100% over 49bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG3783251.567557751031845No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCAGATCGGAAGAGCACACGTCTG2544441.0542672686276267Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCAGATCGGAAGAGCACACGTCTGA2325670.9636217629927262Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT2114920.8762992767626432No Hit
GTCTAGGGGTATGATTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTC1849120.7661672870119621Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA1772410.7343831450489269Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC1752750.7262371897498359No Hit
GTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGAACTCC1431100.5929642232212181Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTATAGTGGTGAGTATCCCAGATCGGAAGAGCACACGTCTGAACTCCAGT1312390.5437777352479174Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG1082890.4486863445489658Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT1063780.44076827711429495Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG989920.41016500863052774Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC984520.40792756414349357No Hit
GTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGAACTCCAGT959280.3974695828744673Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG954070.395310863286051No Hit
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTC940610.38973382573133253Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT925490.38346898116763695Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGT835910.3463522631771703No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTG736270.3050672689756735Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG717230.2971782054510197No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG687400.28481839636801437No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATGTCGTATG668640.27704534848342904TruSeq Adapter, Index 9 (97% over 49bp)
CCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC640850.2655307962066366Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTATAGTGGTGAGTATCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC634940.26308203751804926Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTATAGTGGTGAGTATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA595270.24664510736978168Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC586760.24311906059484453No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC539630.22359114232189645No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT533480.2210429416561075Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA508080.21051866573561354Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC479210.19855662456141426No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACG461240.1911109065184506No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA454590.1883555350668252No Hit
CGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG448820.18596478419827206Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGA431100.17862265154822665Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT429780.1780757206736183Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTG428920.17771938692197953Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCTGAACT414310.1716658565598371Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG406600.16847128304223835No Hit
TGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAG396410.16424914242689057Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCCGCCCCTTGCCTCTCGGCGCCCAGATCGGAAGAGCACACGTCTGAAC395600.16391352575383544Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTAGCGGTTAGGATTCCTAGATCGGAAGAGCACACGTCTGAACTCCAG386680.16021759893451235Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCCGCCCCTTGCCTCTCGGCGCCCCAGATCGGAAGAGCACACGTCTGAA374790.1552910776473205Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC373410.15471928627841175No Hit
CCCCGCCCCTTGCCTCTCGGCGCAGATCGGAAGAGCACACGTCTGAACTC369000.15289203994733389Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA366350.15179403478240044No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCT364670.1510979409419898Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACA359720.1490469501622085No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC357170.14799037915444238No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG354580.14691723448380933Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTG342800.1420362907695015Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG340540.14109987881752054No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG331710.13744124273964803No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC330980.13713877339232675No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGT323440.13401463794191237No Hit
TGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTGA313120.1297386329222471Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA299020.12389641676165793Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGAA299000.12388812993022447Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGAAGATCGGAAGAGCACACGT298590.12371824988583854No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATG292540.12121148337721693TruSeq Adapter, Index 9 (97% over 49bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC287230.11901132963163334No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTGA286220.11859284464424363Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCTGAACTC286210.1185887012285269Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTC283760.11757356437792808No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTCT279020.1156095853281981Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC276270.11447014600609737No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCTG274970.11393150196292248Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGT274770.11384863364858788No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACG269030.11147031302718492No Hit
CGGTGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCC268160.1111098358598294Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTG267990.111039397792645Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTG267850.11098138997261077Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGAACTCCA263460.10916243047296634Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTC261540.1083668946553542Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGA260110.10777438620786182Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTC259550.10754235492772496No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCAGATCGGAAGAGCACACGTCTGA256950.10646506684137517Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGT254310.10537120509215847No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGATCGGAAGAGCACACGTC253320.10496100693620221No Hit
ATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGTCTGAA247240.10244181018043041Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTCGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGA245990.10192388321583919Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT242820.1006104204336358Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCGCCCCTTGCCTCTCGGAGATCGGAAGAGCACACGTCTGAACTCCAG242760.1005855599393354Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)