FastQCFastQC Report
Sat 23 Sept 2023
SRR18884288_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18884288_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69182976
Total Bases5.8 Gbp
Sequences flagged as poor quality0
Sequence length85
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG19693332.8465572224010716No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG8842071.2780702003914952TruSeq Adapter, Index 8 (100% over 49bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT7261791.0496498444935356No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCAGATCGGAAGAGCACACGTCTG7057511.020122349174456Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC6317170.9131104738830547No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCAGATCGGAAGAGCACACGTCTGA4821160.6968708602532507Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG4011230.5798001519911488No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG3133820.45297559908379775No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC3087270.44624706517395263No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT2829220.40894742660390904Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCT2694820.389520682082251Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT2342380.3385775136357245Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC2308670.3337049276400021No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC2070490.29927738292148637No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTGGTATG1906730.27560681980491847TruSeq Adapter, Index 8 (97% over 49bp)
GGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTG1902380.2749780524041059Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC1847510.2670469105000629No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA1823390.2635605036707296Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC1793980.25930945786431625No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA1657870.2396355427092353No Hit
CGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG1614250.23333052339350074Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG1535080.22188695669871153Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG1413980.20438265043700923No Hit
ATGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTG1401430.20256862034960738Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC1381320.1996618358828623No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGT1366960.19758618073903036No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCAC1347950.1948383949253643No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACA1332140.1925531506479282No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGT1296480.18739870340356563No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACG1293360.18694772540574145No Hit
GGGGATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTG1290840.18658347394596034Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA1275300.18433725661064362No Hit
TGCGTGCATTTATCAGATCAAAACCAAAGATCGGAAGAGCACACGTCTGA1271640.18380822472858063Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1268130.1833008744810284No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC1265620.18293806846354801No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA1194720.17268988255145312No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC1191620.17224179543823037No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGATCGGAAGAGCACACGTC1133760.1638784662862725No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACACGT1132820.16374259471000496No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAAGATCGGAAGAGCACACGT1111630.16067970247478225No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGAGATCGGAAGAGCACA1096570.15850286636990002No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTG1073990.15523905765487742Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCT1060660.1533122830680195Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTC1042230.1506483329077951No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGAAGATCGGAAGAGCACACGT1038200.15006581966060553No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTCT1007830.14567601139332312Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT1004790.14523659693390467Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAGATCGGAAGAGCA1002420.14489402710863436No Hit
GGGGATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGT988600.14289642584904125No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG987950.14280247209949454No Hit
ATGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGA959300.13866127990793572Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACAC956240.13821897456391583No Hit
GGGGATGCGTGCATTTATCAGATCAAAACCAGATCGGAAGAGCACACGTC944010.136451198630137No Hit
GGGAGACCGGGGTTCAATTCCCCGACGGGAGATCGGAAGAGCACACGTCT925430.13376556683540183Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCAC918530.13276821164790598No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTATTAGATCGGAAGAGCACAC917210.13257741326421113No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC904990.13081108277273298No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA882320.12753426507700394No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCA861820.12457110836053079No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCAGATCGGAAGAGCACACGTCTGA847140.12244919906307587Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGT841470.12162963327856842No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC831190.12014371859342969No Hit
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTC820490.1185970953316608Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGT820360.11857830458175145No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA818500.11830945231381777No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACA816590.11803337283438052No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACACG799110.1155067396927244No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCAGATCGGAAGAGCACAC787380.1138112358739815No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACG765850.11069919860053432No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC756470.10934337372246028No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTG755660.10922629289610206Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA747060.10798321251748408Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGCGCAATGAAGGTGAAGGGCCCCAGATCGGAAGAGCACACGTCTGA733480.10602030187310821Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCAGATCGGAAGAGCACACG714110.10322048013661628No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT713550.10313953536777604No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGAGATCGGAAGAGCACACGT710530.10270301179295901No Hit
GGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGT700300.10122432431932386No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)