FastQCFastQC Report
Sat 23 Sept 2023
SRR18915702_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18915702_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147214259
Total Bases22 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC7239640.4917757321320349No Hit
TTTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGT6044440.4105879444735038No Hit
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTA4874950.3311465909019044TruSeq Adapter, Index 10 (100% over 47bp)
TTTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT4780670.32474231996779607Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACAC4497470.30550505301256176No Hit
GTTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC4243700.2882669130576543No Hit
ACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTA4239800.28800199306780466TruSeq Adapter, Index 10 (100% over 47bp)
TTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTA3565760.2422156674374865TruSeq Adapter, Index 10 (100% over 47bp)
GTTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGT3514180.23871193075121888No Hit
TTCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC3373650.22916598045030406No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC3148420.21386651139547563No Hit
ATTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC2860210.19428892414558838No Hit
GTTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT2807460.1907057114623659Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTA2706930.18387688926247286TruSeq Adapter, Index 10 (100% over 47bp)
CTTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC2663770.18094510804147038No Hit
CTTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGT2432380.16522720125908458No Hit
ATTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGT2391320.1624380692633857No Hit
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTA2368540.16089066480985378TruSeq Adapter, Index 10 (100% over 47bp)
TCTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGT2352440.1597970207491925No Hit
TTCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACA2339290.1589037648859816No Hit
TTTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA2287830.15540817958401706No Hit
TTCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGAGATCGGAAG2163020.1469300606268038No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACAC2114280.14361924003570878No Hit
GCTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC2088050.1418374832834637No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT2058920.1398587347438946Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC1982910.13469551206992794No Hit
CTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACAC1942610.1319580055081485No Hit
ATTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT1853180.12588318635628903Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTA1795820.12198682465942379TruSeq Adapter, Index 10 (100% over 47bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC1764160.11983621776746503TruSeq Adapter, Index 10 (100% over 50bp)
CTTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT1754260.11916372856246216Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGT1726360.11726853171199944No Hit
TTTCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGAGATCGGAA1695000.11513830328079837No Hit
ATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTA1688670.11470831775881167TruSeq Adapter, Index 10 (100% over 47bp)
TCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACAC1688440.11469269427223079No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGT1649230.1120292294512042No Hit
ATCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACAC1643970.1116719271059198No Hit
TGTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC1638400.11129356701785253No Hit
TATCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC1610810.10941942791017276No Hit
GTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTA1555040.10563107205532313TruSeq Adapter, Index 10 (100% over 47bp)
GTCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC1480900.10059487512007924No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)