FastQCFastQC Report
Sat 23 Sept 2023
SRR18915703_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18915703_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107600368
Total Bases16.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC5691470.5289452169903359No Hit
ACGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC3605810.33511130742601175No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC3501440.32541152647358973No Hit
TTTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGT3280570.3048846450041881No Hit
GTTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC3177480.29530382275272515No Hit
TTTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT2873650.2670669304774125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC2830730.263078096535878No Hit
ATTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC2271800.21113310690535927No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC2179470.20255228123383368No Hit
CTTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC1962900.18242502665046648No Hit
TTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACA1874590.17421780564914052No Hit
GCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC1837610.17078101442924434No Hit
GTTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGT1832320.17028938042293684No Hit
TTTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGC1815830.16875685778323732No Hit
CTGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC1665920.15482474929825518No Hit
ATGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC1637240.1521593309048906No Hit
GTTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT1627480.15125227081007753Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC1617690.15034242262071074No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC1396510.12978673084092054No Hit
CTTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGT1360460.12643637055218993No Hit
ATTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGT1334130.1239893528988674No Hit
GCTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC1281560.11910368187588354No Hit
GTGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC1218250.11321987300266481No Hit
TTTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCAC1212820.11271522788843993No Hit
ATTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT1193450.11091504817158247Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT1118610.10395968162488069Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGT1088290.10114184739591224No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)